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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.1

Comparison of Testis Proteins between Adult Yak and Bisexual Yak by Two-dimensional Electrophoresis
Author of the article:FU Wei1, LI Caixia1, HUANG Lin1, LIU Wenjing2, JIN Suyu1, LIN Yaqiu1, ZHENG Yucai1*
Author's Workplace:(1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China; 2. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan Province 621010, China)
Key Words:yak; bisexual yak; testis; two-dimensional electrophoresis; mass spectrometry
Abstract:The purpose of this study was to compare the differently expressed proteins in testes of adult yak and bisexual yak, and provide evidence for further exploring the molecular mechanism of infertility of bisexual yak. Total proteins were extracted from the testes of normal yaks (n=4) and bisexual yak (n=1), and then separated by two-dimensional electrophoresis followed by identification of the differently expressed proteins by mass spectrometry. The well-resolved, reproducible two-dimensional electrophoresis gels of adult yak and bisexual yak testis protein were obtained. The result showed that there were 24 protein spots with two fold expression difference, and 22 of which were identified after searching in the databases. Six proteins were molecular chaperones and the expression were significantly decreased in the testis of bisexual yak, which may play an important role in spermatogenesis disorder.
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