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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.1

Construction of (AC)n and (AG)n Microsatellite DNA Libraries in Pantholops hodgsonii
Author of the article:DU Yurong1, MA Jianbin1, XU Yongtao1, ZHOU Zhihong1, TAN Chunmin1, ZHANG Xuze2, GUO Songchang 2,3*
Author's Workplace:(1. School of Life and Geography Science, Qinghai Normal University, Xining 810008, China; 2. Key Laboratory of Evolution and Adaptation of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; 3. Key Labratory of Crop Molecular Breeding of Qinghai Province, Xining 810007, China)
Key Words:Pantholops hodgsonii; microsatellite; enriched-library
Abstract:Tibetan antelope (chiru, Pantholops hodgsonii) is an indigenous species of Qinghai-Tibetan Plateau and listed as an AppendixⅠspecies in CITES and Category Ⅰ in the Key Protected Wildlife List of China. Microsatellites and SSR are powerful molecular markers of numerous biological contexts. In this study, the microsatellite enriched-libraries of the chirus were constructed according to the FIASCO (Fast Isolation by AFLP of Sequences Containing repeats) protocol. Finally, 76 and 61 positive clones were screened in 150 white clones picked from (AC)n and (AG)n microsatellite libraries, respectively. Based on the result of sequencing, 37 SSR were detected from (AC)n library and 29 from (AG)n library among the positive clones. A total of 15 microsatellite loci were identified to be polymorphic using 47 designed primer pairs. These may facilitate further studies on the genetic diversity, genetic structure, demographic history and genetic linkage mapping of Tibetan antelopes.
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