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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.1

Cloning, Sequence Analysis and Expression of PL10 Gene in White Shrimp, Exopalaemon modestus
Author of the article:SHI Taodan, WU Ping*, YE Yuantu, WANG Min, Wei Yuhong
Author's Workplace:(School of Biology and Basic Medical Sciences, Soochow University, Suzhou, Jiangsu Province 215123, China)
Key Words:Exopalaemon modestus; PL10; cloning; cDNA; tissue distribution
Abstract:The PL10 gene of white shrimp (Exopalaemon modestus) encodes an ATP-dependent RNA helicase which belonging to the DEAD box family. In this study, a full-length cDNA sequence of the PL10 gene was cloned from the ovaries of white shrimp by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. And the structure and speculated function of PL10 were analyzed. The full length of Em-PL10 was comprised of 2559 nucleotides with a3’untranslated region (UTR) of 318 bp, a5’UTR of 87 bp and an open reading frame (ORF) of 2154 bp, encoding 717 amino acids. The putative amino acid sequence shared eight conserved motifs of the DEAD-box family and GG doublet. And there were five arginine-glycine-glycine (RGG) repeats which might be functioned as RNA-binding in the N-terminal portion. Sequences comparison revealed that the deduced amino acid sequence of Em-PL10 showed high similarity to the PL10 homologs of Macrobrachium nipponense (92%), Fenneropenaeus chinensis (81%) and Drosophila melanogaster (64%). Subcellular localization analysis showed that the PL10 protein of E. modestus was located in nucleus. In addition, phylogenetic analysis based on amino acid sequences of PL10 suggested that E. modestus was closely related to M. nipponense. Furthermore, tissue expression of Em-PL10 was detected by semi-quantitation RT-PCR. The results showed that the expression of Em-PL10 could be detected in variant tissues, and the highest expression level was detected in ovaries followed by testes. The RT-PCR results also indicated that there was little expression of this gene in thoracic ganglions, gills and hepatopancreas.
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