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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.1

Construction of Stella Eukaryotic Expression Vector and Its Effect on Pluripotency of Mouse Embryonic Stem Cells
Author of the article:BAI Yaofu1#, LIU Weishuai2#, HUA Jinlian1*
Author's Workplace:(1. College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Key Lab for Animal Biotechnology of Ministry of Agriculture of China, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province 712100, China; 2. Pathology Department of Yangling Demonstration Zone Hospital, Yangling, Shaanxi Province 712100,China)
Key Words:embryonic stem cells; meiosis; pluripotency
Abstract:To explore the effects of recombinant vector Stella on the pluripotency of mouse embryonic stem cells (ESCs) and expression profile of specific meiosis marker-Stra8 in transfected ESCs. The eukaryotic expression vector containing Stella was constructed and transfected into ESCs. Expression profiles of GFP was observed in transfected ESCs, and expression levels of pluripotent genes and specific meiosis markers were simultaneously detected by RT-PCR and immunofluorescent staining. The results demonstrated that although Stella which had been cloned in the recombinant vector was successfully expressed in ESCs as identified by GFP and PCR amplification, it had no significant effect on the expression profiles of pluripotent and specific meiosis marker genes.
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