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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.6

The Effect of Polymorphism K232A of DGAT-1 Gene on Milk Production Trait in Chinese Holstein Cattle
Author of the article:MA Yannan1,2, HE Pengjia1 , MA Yongsheng1, DONG Yanjiao1, ZHU Jing1, LEI Zhaomin2, LIU Zhe2, WU Jian
Author's Workplace: (1. Department of Chemistry and Life Sciences, Gansu Normal University for Nationalities, Hezuo, Gansu Province 747000, China; 2. Animal Science and Technology, College of Gansu Agricultural University, Lanzhou 730070, China)
Key Words:Chinese Holstein cattle; diacylglycerol acyltransferase (DGAT)gene; K232A; milk traits
Abstract:

To investigate the association between the polymorphism of DGAT-1 gene and milk yield and composition, 232 Chinese Holstein cattles in Gansu region were collected to study the polymorphism of the K232A site of DGAT-1 gene using PCR-SSCP in this study, and the effects of the polymorphism of the K232A site on 305 days milk yield, milk fat yield and milk protein yield were analyzed based on a mixed animal model. Three genotypes including KK, KA and AA were identified with genotypic frequencies 0.5086, 0.3750 and 0.1164, respectively. Additionally, the gene frequencies of K and A were 0.6961 and 0.3039, respectivelyThe polymorphism information content (PIC) was 0.3336, and the population was in accordance with Hardy-Weinberg equilibrium at this locus. Significant association was observed between the K232A site and 305 days milk yield (P<0.01), milk fat yield (P<0.05) and milk protein yield (P<0.05). The result of Least Squares analysis showed that 305 days milk yield of AA and KA types was significantly higher than that of KK type (P<0.01); 305 days milk fat yield of KK type was significantly higher than that of AA and KA types (P<0.05); and 305 days milk protein yield of AA type was significantly higher than that of KK type (P<0.05). Allele A was the dominant allele of high milk yield and milk protein yield, while K was dominant allele of high milk fat yield. Therefore, these genotypes can be considered as the molecular genetic markers of the breeding of Chinese Holstein cattle in Gansu region.

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