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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.6

Nucleotide and Predicted Protein Structure Analysis of MC4R Gene of Yak
Author of the article:CAI Xin1, TserangDonko Mipam 2
Author's Workplace:(1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan Province 621010, China;
Key Words: Bos grunniens; MC4R gene; sequence analysis; protein structure prediction
Abstract:

This work was aimed to clone the MC4R gene of yak (Bos grunniensand analyze its structure and predict the protein structure. Results of this work were expected to provide basic data for further study of the roles of MC4R gene on body weight controlling, restricted feeding weight, fat deposition and energy balance of yak. Homologous primers were designed based on bovine MC4R gene to clone yak MC4R gene. Bioinformatics analysis was conducted to detect the variation of yak MC4R gene and predict the protein structures. Compared with the MC4R gene of bovine, five polymorphic sites was detected in yaks and three were located at the third nucleotide of the genetic code and two at the first. Among the four yak individuals, only two polymorphic sites were detected at the third nucleotide of the genetic code. We predicted that the two amino acid substitutions might lead to slight changes in the secondary structure of MC4R, and the protein structure of MC4R was highly homologous between yak and cattle, suggesting that MC4R gene might have been subjected to substantial constraints or strong purifying selection during the several million years of evolution.

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