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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.5

The Difference of the Patterns of H3K27 Acetylation between Parthenogenetic Embryos and in vivo Mouse Embryos and the Effect on Development
Author of the article:WANG Caihong1, LIU Yong2,3, HUANG Jichang1, WU Fengrui2,3, DING Biao2,3, ZHANG Yuan2, WANG Rong3, LI
Author's Workplace:(1. College of Life Science, Anhui University, Hefei 230039, China; 2. Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, Fuyang, Anhui Province 236041, China; 3. College of Life Science, Fuyang Normal University, Fuyang, Anhui Province 236041, China)
Key Words:mouse; oocyte; parthenogenetic embryos; embryonic development; H3K27 acetylation
Abstract:

To investigate the difference of the patterns of H3K27 acetylation between parthenogenetic embryos and in vivo mouse embryos, and explore the effect of epigenetic mode on parthenogenetic embryos development. Method Different period of parthenogenetic embryos were obtained by using strontium chloride activated mouse oocytes, and the developmental rates of these embryos were counted. After superovulation by injecting pregnant mare serum gonadotrophin and human chorionic gonadotropin, different period of embryos were collected by washing of uterus in vivo. These embryos were labeled by H3K27 acetylated antibody and then incubated with FITC fluorescent secondary antibody. The relative fluorescence intensities of these reprocessed parthenogenetic embryos and in vivo mouse embryos were detected by laser scanning confocal microscope. Result The activation rate and blastocyst rate of parthenogenetic embryos were 96.385±0.385 and 69.54±2.87, respectively. The fluorescence intensity of H3K27 acetylated parthenogenetic embryos performed a relatively high level at first and then significantly reduced during the periods of 2-cell, 4-cell and 8-cell. The level of the fluorescence intensity increased rapidly in morula. The overall trend of the fluorescence intensity in parthenogenetic embryos was similar with in vivo. The relative fluorescence intensity of H3K27 acetylated parthenogenetic embryos was higher than in vivo from prokaryotic period to 8-cells, whereas the others were opposite. Additionally, significant differences were observed between parthenogenetic embryos and in vivo embryos in all periods (P<0.01) except in prokaryotic period and morula period (P>0.05). Conclusion This study shows that the patterns of H3K27 acetylation in parthenogenetic embryos are different with in vivo mouse embryos, and this might be an important reason affecting the developmental ability of parthenogenetic embryos. Further study will be important for correcting the acetylation patterns and improving the viability of parthenogenetic embryos.

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