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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Construction and synergistic effect of recombinant yeast co-expressing porcine IL-2 and fusion antimicrobial peptide gene on immunity and growth of mice
Author of the article:HU Bing1#, WU Xueying1#, MA Changjun1#, WAN Xiaoping1, XIAO Yongle1, CHEN Jianlin1, LI Jianglin2, LV
Author's Workplace:(1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry Education, Key Laboratory of Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Sichuan Academy of Animal Science, Chengdu 610066, China; 3. Center for Animal Disease Control of Sichuan Province, Chengdu 610035, China)
Key Words: porcine IL-2; fusion antimicrobial peptide; recombinant Pichiapastoris; mice; immunity
Abstract: To develop a novel and practicable immunomodulator, the recombinant Pichiapastoris to co-express porcine IL-2 and fusion antimicrobial peptide gene were constructed by 2A self-cleavage technique, and then fermented to feed ICR mice by gavage to evaluate its effects on the immunity and growth of mice via real time fluorescent quantitative RT-PCR, ELISA and flow-cytometry. It was found that the expressed molecules of recombinant yeasts not only showed remarkable immunological bioactivity but also manifested obvious antimicrobial activity in vitro. The feeding of the yeast relatively improved the growth of mice in comparison to the control group (P<0.05). The contents of IgG, IgG1 and IgG2a were significantly increased in the yeast-treated groups (P<0.05), and the expression levels of the TLR1, TLR4, TLR6, TLR9, IL-7, IL-15, IL-23, CD62L, IL-2, IL-4, IL-6, IL-12, CAMP and CRP4 genes significantly increased in comparison to the control (P<0.05). Similarly, significant increases of the leukocytes, Th and Tc cells were detected in the blood of yeast-treated mice (P<0.05). Meanwhile, the mice of yeast-treated groups displayed markedly higher levels of immunity and survival rates than that of the control after challenged with virulent bacteria Escherichia coli and Staphylococcus aureus (P<0.05). These results suggest that the recombinant yeast can effectively enhance the immunity and growth of mice, and thus can be further developed as a promising safe and effective immunomodulator for livestock.
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