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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Comparison of gene expression of Toll-like receptors and antimicrobial peptides in immune organs and tissues of adult Chenghua, Wujin and Qingyu pigs
Author of the article:Zhang Jingmei
Author's Workplace:Sichuan University
Key Words:pig; TLRs gene; antimicrobial peptides; gene expression; Immune organs and tissue
Abstract:Toll-like receptors (TLRs) and antimicrobial peptides are important components of innate immunity of the natural immune system and an important barrier to protect against harmful microorganisms or viruses. Chenghua pig, Wujin pig and Qingyu pig are well known Chinese unique local subspecies of pig breed, and they own the merits of high fertility and strong disease resistance. In this study, we compared the innate immunity characteristics of 8-month-old Wujin pigs, Qingyu pigs, Chenghua pigs and Yorkshire pigs with local immune tissues and systemic immune organs. Real-time fluorescent quantitative PCR was used to detect the expressions of TLRs (TLR2, TLR4, TLR7 and TLR9) and antimicrobial peptides (pBD-1 and PR-39) genes in thymus, spleen, amygdala, mesenteric lymph node and hilar lymph nodes. The results showed that compared to the control pigs,the expression levels of TLR7 and PR-39 in thymus, TLR4 and TLR7 in spleen, TLR4 and TLR9 in mesenteric lymph nodes, and TLR1, TLR4, TLR7 and TLR9 mRNA in hilar lymph nodes of Chenghua pigs were significantly higher (P<0.05); The expression level of TLR1, TLR9 in the thymus, TLR2, TLR4, TLR7, TLR9 and pBD-1 in the tonsils, TLR7 in the mesenteric lymph nodes of Wujin pig were significantly higher (P<0.05); The expression level of PR-39 mRNA in the tonsils and mesenteric lymph nodes of Qingyu pigs was higher than other pig breeds (P<0.05). These results provide useful clues for screening of disease-resistant molecular markers of local pig hybrid breeding in the future.
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