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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Cloning and Functional Analysis of Caspase-7 Gene from Dugesia japonica
Author of the article:Zhihong Wang
Author's Workplace:College of Life Sciences, Sichuan University
Key Words:Dugesia japonica; Caspase-7; Gene cloning; Tissue remodeling
Abstract: In this paper, the whole length of the Caspase-7 gene of Dugesia japonica was first cloned using the RACE technique. The Caspase-7 gene of D.japonica is 923 bases in length, encodes 251 amino acids, and contain 86 and 81 bases of the 5'-UTR and 3'-UTR regions, respectively. It also contains two key conserved domains of Caspase family, LSHG and QAC (Q/R)G. The phylogenetic analysis reveals that planarian Caspase-7 has closer relationship with that of Hydra vulgaris than the Caspase-7 from other species of Platyhelminthes. Besides, the predicted protein three-dimensional structure of planarian Caspase-7 showed a very high similarity with bacterial Caspase-7 protein. In addition, qPCR technology was used to evaluate the expression of Caspase-7 at different time of planarian regeneration, and the result revealed that Caspase-7 may be involved in the regulation of apoptosis in planarian. In addition, knocking down the in vivo expression of Caspase-7 by feeding dsRNA interference in the planarian did not influence the regeneration of planarian, but led to a significantly decrease in the expression of Caspase-3-like-gene and PCNA, suggesting apoptosis and proliferation were significantly reduced. Finally, the regenerative or untreated planarians were dissolving under the starvation condition due to Caspase-7 interference-induced an imbalance of remodeling, implying Caspase-7 plays a key role in planarian tissue remodeling.
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