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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Population genetic structure and geographic differentiation of Saurogobio punctatus (Teleostei, Cypriniformes, Cyprinidae) in Yangtze River basin based on cytochrome b gene sequences
Author of the article:LI Xiaobing, TANG Qiongying,YU Dan, LIU Huanzhang
Author's Workplace:1. The Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences 2. University of the Chinese Academy of Sciences
Key Words:angtze River basin; Saurogobio punctatus; Cyt b; Genetic structure; Geographic differentiation
Abstract:The population genetic structure and geographic differentiation process of three Saurogobio punctatus populations from the Chishui River (one branch of the Upper Yangtze River), the Upper Yangtze River main drainage, and the Middle Yangtze River were studied based on mt DNA cytochrome b gene sequences of 303 individuals. The analyzed cytochrome b gene sequences were 1097 base pairs in length, with 80 variable sites including 34 parsimony informative sites. 49 haplotypes were identified from 303 individuals, which showed high haplotype diversity (Hd=0.803) and low nucleotide diversity (Pi=0.00371) respectively. Based on haplotype dataset, phylogenetic trees constructed using NJ, ML and BI methods showed that all haplotypes from the Middle Yangtze River cluster together and form a monophyletic group, which locates in the derived position of the phylogenetic trees, whereas haplotypes from the Upper Yangtze River main drainage and the Chishui River cannot form a monophyletic group having relative basal positions in the phylogenetic tree. Extensive gene flow might exist between populations in the Upper Yangtze River main drainage and Chishui River since many haplotypes were shared by them with a low genetic differentiation index (FST=0.0294) between them, suggesting a close relationship. The FST values between the Middle Yangtze River and Chishui River populations, between the Middle Yangtze River and the Upper Yangtze River main drainage population are 0.614 and 0.706, respectively, suggesting a high level differentiation. Neutral test and mismatch analysis showed that both the Upper Yangtze River main drainage and Chishui River populations have experienced population expansion, but not in the Middle Yangtze River population. BSP (Bayesian skyline plot) analysis shows that Saurogobio punctatus populations began expansion from 0.20 Ma BP (Millions year before present), and lasted until the end of the last interglacial period, the Marine Isotope Stage (MIS) 5, then experienced a rapid expansion. According to BSP analysis and haplotype network, we inferred that the origination center of Saurogobio punctatus population lied in the Upper Yangtze River, and then gradually spread to the Middle Yangtze River via population expansion, and eventually evolved into a distinct genetic population.
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