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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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DNA barcoding of Bambusa emeiensis-feeding leafhoppers in China for species identification
Author of the article:YAO Yalin, LUO Qiang, DONG Mengshu, CHEN Xiangshen
Author's Workplace:1. College of Foresty, Guizhou University, Guiyang 550025, China; 2. Special Key Laboratory for Development and Utilization of Insect Resources of Guizhou Universty, Guiyang 550025, China; 3. Institute of Entomology, Guizhou University, Guiyang 550025, China; 4. Key laboratory for Plant Pests Management of Mountainous Region in Guizhou Province, Guiyang 550025, China
Key Words:DNA barcoding, COI, 16S rRNA, species identification, leafhoppers, Bambusa emeiensis
Abstract:Leafhoppers play a key role in biodiversity and plant protection of agricultural and forestry ecosystems. However species identification is challenging for this group due to their morphology diversity. DNA barcoding provides an efficient method for species identification. In the present study, the partical COI and 16S rRNA gene sequences from 12 bamboo-feeding leafhopper species in China were amplified. A neighbor-joining phylogenetic tree was constructed based on Kimura 2-parameter genetic distance, further studied using Klee- diagram vector analysis. Intra-specific K2P genetic distance averaged 0.004 for COI, and 0.003 for 16S rRNA; inter-specific K2p genetic distance averaged 0.283, and 0.257 for COI and 16S rRNA, respectively. An obvious ‘barcoding gap’ was detected for all species in both markers and all individuals of a species clustered together in both phylogeny trees of the COI and 16S rRNA. The result of cluster analysis, based on COI and 16S rRNA gene sequences were basically consistent with the result of morphological identication, different individuals of the same specieses were gathered to a branch in the dendrograms, Klee- diagram vector analysis get the same result with phylogeny. Hence, both COI and 16S rRNA appear to be accurate and efficient markers for bamboo leafhoppers identification in China.
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