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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Establishment of an orthotopic lung cancer model by injection of EMT-6 cells
Author of the article:WEI Shihang,WEI Shiyuan,SHI Xueni,PENG Xu,HE Xulin
Author's Workplace:College of Life Science,Sichuan University; College of Life Science,Sichuan University; College of Life Science,Sichuan University; Sichuan University, Laboratory Animal Centre; Sichuan University, Laboratory Animal Centre; Sichuan University, Laboratory Animal Centre; Sichuan University, Laboratory Animal Centre;
Key Words:EMT-6; different concentrations;animal model of metastasis lung cancer;
Abstract: Objective: To establish a metastasis lung cancer model, characteristics is consistent with clinical, stable and repeatable, in BALB/c mice by intravenous injection of EMT-6 breast-tumor cells. Methods: Harvest the logarithmic growth phase cells of EMT-6 murine mammary carcinoma by 0.25% trypsin digestion, and equilibrated at 3 density of 1*106 cells per 1ml(high), 5*105 cells per 1ml(medium) and 1*105 cells per 1ml(low) in phosphate-buffered saline(PBS). And then intravenously inject 0.2ml EMT-6 tumor cells. Do research on evaluation indicators and biological characteristics such as clinic appearance, the time of tumor formation, survival time, the tumor growth and pathological characteristics of metastasis lung models with different concentrations. And then screen the best metastasis lung cancer model. Finally determine the repeatability and stability of the model by 3 repeated experiment, successfully established a metastasis lung cancer model in BALB/c mice. Results: Anatomized BALB/c mice after EMT-6 tumor cells injection, it showed that in the group of high concentration (1*106/ml), tumor nodes on lung surface began to appear at 7d and all animals died in 18d; in the group of medium concentration(5*105/ml), tumor nodes on lung surface began to appear at 7d and all animals died in 28d; in the group of low concentration(1*105/ml), tumor nodes on lung surface appeared partially at 14d, all mice lung surface had tumors at 21d, the number of tumors reach the highest level(an average of 12-15/mouse) and volume of tumors became much bigger at 35d, mice began to die at 28d, and all mice died in 42d. We concluded that established model with the low EMT-6 cell concentration can provide suitable 4weeks time window for treatment , observation and test from tumor formation to dead all, and the histopathology of models’ lung was qualified with clinic features of lung cancer, clinic appearance was easy to identify , repeatability and stability test showed the models were consistent among the three times. This is more useful model for metastasis lung cancer research work.
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