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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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The Role of the Sperm-specific Lactate Dehydrogenase (LDH-C4) in Plateau Pika Tissues
Author of the article:WANG Zhijie, WANG Yang, AN Zhifang, WEI Linna, WEI Lian, WEI Dengbang
Author's Workplace:Qinghai University
Key Words: Plateau pika (Ochotona curzoniac); tissues; Ldh-c gene; LDH enzymatic activity; lactic acid; ATP
Abstract:The plateau pika (Ochotona curzoniac) has a strong adaptability to a hypoxic plateau environment .Previous studies found that the sperm-specific lactate dehydrogenase (LDH-C4) gene is expressed in plateau pika somatic cell. In order to shed light on the role of LDH-C4 in plateau pika tissues, we silenced Ldh-c gene in pika heart ,liver and brain by RNA interference. We determined the expression levels of Ldh-c gene in plateau pika heart, liver and brain by real-time PCR and western blot. Also by using the biochemical method to measure the activities of LDH, and the contents of lactic acid and ATP in plateau pika heart, liver and brain after silencing Ldh-c gene expression. The results indicated that by intraperitoneal injection of adenovirus pMultiRNAi-Ldhc could significantly reduce the expression of Ldh-c in tissues of plateau pika, in the Ldh-c mRNA and protein level, the expression levels of Ldh-c in heart were decreased by 48.11% and 19.27%; the expression levels of Ldh-c in liver were decreased by 70.16% and 25.82%; the expression levels of Ldh-c in brain were decreased by 49.08% and 25.36%. At the same time it can significantly reduce the activities of LDH, and the contents of lactic acid and ATP after silencing Ldh-c gene expression, decreased by 25.58%, 41.94% and 21.23%; 28.16%, 15.90% and 24.66%; 16.65%, 12.78% and 18.50%, respectively. These results suggest that the plateau pika in severe hypoxia in the heart, liver and brain by LDH-C4 catalyzed anaerobic glycolysis was part of ATP and enhance the adaptation to the hypoxic environments.
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