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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Study on genetic diversity and genetic structure of Rhizomys pruinosus
Author of the article:DING Xuemei
Author's Workplace:Southwest Forestry University, Qujing Normal University
Key Words:Rhizomys pruinosus; recombination activating protein 1 gene; genetic diversity; genetic structure; Yunnan-Guizhou plateau
Abstract:As a subterranean fossorial rodent, Rhizomys pruinosus has high edible and medicinal value, and has become the important animal species for the development of special economic animal breeding industry in South China. By using molecular biological methods, this research intends to adopt a molecular marker of nuclear recombination-activating gene 1 (RAG1) to investigate the population genetics of 173 individuals from 42 sampling sites, and the genetic diversity and phylogeographical structure of R. pruinosus. The analyses of sequence data polymorphism of R. pruinosus RAG1 gene showed that there were 18 polymorphic sites in total 848 locus, including 3 single mutation sites and 15 parsimony informative sites. The results of genetic diversity analyses discovered that there were 11 haplotypes from 173 individuals’ estimation. The haplotype diversity and nucleotide diversity were 0.712 ± 0.025(SD) and 0.00264 ± 0.00371(SD) respectively, which were all significantly lower than that of the mitochondrial gene. The phylogenetic trees constructed by the methods of maximum likelihood and neighbor-joining showed that R. pruinosus groups diverged to three clades, which meant obvious differentiation happened in the phylogeographical pattern of this species. In addition, the results of molecular variance analyses (AMOVA) approved that the genetic variation between populations of R. pruinosus was significantly higher than that within populations. This illustrated that there were significant genetic structure and genetic differentiation in total groups of this species. All above results showed comprehensively that low genetic diversity and remarkable genetic structure were detected in R. pruinosus groups, which were related with the important factors of subterranean life style, weak distribution ability, mountain river barrier and geological events. The research findings will provide a scientific basis for the species diversity and biodiversity protection in the Yunnan-Guizhou plateau.
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