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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Molecular Cloning and Tissue Expression of Chicken GPR119-like Receptor Gene
Author of the article:DENG Qiuyang, ZHANG Jiannan, WANG Yajun, LI Juan
Author's Workplace:Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:chicken; GPR119b; molecular cloning; tissue expression
Abstract:

G protein-coupled receptor 119 (GPR119) is a member of class A (rhodopsin-type) G protein-coupled receptor family. In mammals, GPR119 gene is found to be highly expressed in pancreatic islet β cells, PP cells and intestine endocrine L cells involving in insulin secretion. In this study, we cloned a chicken GPR119-like gene (named cGPR119b) from intestine and examined its expression in chicken tissues. The results showed that the full-length cDNA of cGPR119b was 954 bp in length and encode a precursor protein of 317 amino acids. Sequence analysis revealed that chicken GPR119b shares high amino acid sequence identity with that of Anas platyrhynchos (79.9%), Chelonia mydas (61.0%), Chrysemys picta bellii (62.3%), Meleagris gallopavo (95.5%).5’-RACE PCR assay revealed that the5’UTR of chicken GPR119b is 678 bp. Quantitative real-time PCR assay showed that cGPR119b was widely expressed in various chicken tissues including the brain, liver, kidney, intestine, ovary, testis, pituitary, pancreas, skin, fat, lung, duodenum, jejunum, ileum, cecum and rectum. GPR119b is highly expressed in the liver, kidneys, cecum and testes and weakly expressed in the pancreas, skin, fat and lung. Present study provided a basis to uncover the physiological roles of GPR119b in chickens.

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