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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Regulatory molecular mechanism of Periplaneta Americana extraction on Wound Healing using RNA-seq
Author of the article:CHEN Jiasong, CHEN Feng, PENG Rui, ZOU Fangdong
Author's Workplace:College of Life Sciences, Sichuan University, Sichuan Engineering Research Center for Medicinal Animals, Sichuan Key Laboratory for Medicinal American Cockroach
Key Words: Periplaneta Americana; Wound healing; RNA-seq; Key genes
Abstract: Periplaneta americana is a traditional Chinese medicine, and its extracts have been widely used in the treatment of wound in clinic. However, the molecular mechanism is poorly understood. In this study, to investigate the regulatory mechanism of P. americana extracts on wound healing, a mouse model with excision wound was constructed to assess the efficacy of P. americana extracts on wound therapy. A total of 9 mice were randomly divided into three groups: control group (treated with 75% ethanol solution) and two P. americana extracts treated groups (Kangfuxin and Jifeng), respectively. RNA-seq was used to screen the key genes in wound skin tissues on  day 3. Furthermore, real-time quantitative PCR was performed to confirm the expression of key genes. The results showed that both P. americana extracts could accelerate wound healing. RNA-seq analyses showed that, comparison to the control group, a total of 545 differentially expressed genes (DEGs) were identified in Jifeng treated groupand 938 DEGs in Kangfuxin treated group. Bioinformatics analysis revealed that three key genes, including Ereg, Gli2 and Tgm3, may play an important role in wound healing during the treatment of P. americana extracts. Q-PCR experiments further confirmed that Ereg, Gli2 and Tgm3 were up-regulated after the treatment of both P. americana extracts. Collectively, our results indicated that the extracts of P. americana may accelerate wound healing by regulating Ereg, Gli2 and Tgm3 expression.
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