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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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nhancement of immune response to PCV-2 vaccine by porcine IL-2 and fusion IL-4/6 gene entrapped in chitosan nanoparticles
Author of the article:Yi Chen, Tingyu Song, Yong-Le Xiao, Xiaoping Wan
Author's Workplace:Sichuan University,Sichuan Huapai Biopharmaceutical company, Center for Animal Disease Control of Sichuan Province,
Key Words:Piglet, gene co-expression, IL-2, fusion IL-4/6, immunity, PCV-2 vaccine
Abstract:Objective In order to develop a novel effective immunomodulator to enhance pig resistance against post-weaning multisystemic wasting syndrome (PMWS), we used the recombinant plasmid co-expressing pig interleukin-2 (IL-2) and fusion interleukin-4/6 (IL-4/6) genes we constructed before, studied its effect on immune response of piglets. Methods The recombinant plasmid was encapsulated in chitosan (CS) nanoparticles prepared by the ionotropic gelation method, designated VRIL4/6-2-CS. Then piglets were divided into two groups and intramuscularly injected respectively with VPIL4/6-2-CS and saline along with the PCV-2 (pig circovirus-2) vaccine. The blood was collected from each piglet on days 0, 7, 14 and 28 after vaccination to assay the immunological changes. Results The number of IgG2a, CD4+, CD8+ T cells increased significantly in the sera or blood of piglets treated with VPIL4/6-2-CS (P<0.05). Furthermore, the expression levels of IL-2, IL-4, IL-6, TLRs (TLR-2, 7) and TNF-α elevated significantly in the treated piglets (P<0.05) over an 28 day observation period. Although there were no significant differences in PCV-2-specific antibody levels, the growth performance of the treated piglets was markedly improved in comparison with controls (P<0.05). Conclusion These indicate that VRIL-4/6-2 entrapped with CS is a promising effective adjuvant to promote the immune response of pig to PCV-2 vaccination.
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