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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Molecular Cloning, Tissue Distribution and Expression of GnRH in Different Developmental Stages of Brandt’s vole (Lasiopodomys brandtii)
Author of the article:LIU Lan, CHEN Yan, LI Ning, et al.
Author's Workplace:College of Life Science, Sichuan UniversityInstitute of Plant Protection, Chinese Academy of Agricultural Sciences
Key Words:Brandt’s vole, GnRH gene, cloning, gene expression, reproductive regulation
Abstract:Gonadotrophin releasing hormone (GnRH) is encoded by GnRH and is the core neuroendocrine substance that regulates the reproductive activity of animals. Brandt’s vole (Lasperodomys brandtii) is one of the rodent species in the grassland of eastern Inner Mongolia, and shows obvious seasonal reproduction, but its reproductive regulation mechanism is still not completely clear. In this study, the GnRH cDNA were cloned from the hypothalamus of Brandt's vole, and the GnRH mRNA of different tissues and different ages were measured using real-time qPCR. The results showed that 497bp of cloned GnRH cDNA sequence contained an open reading frame (ORF) of 273bp, encoding a GnRH precursor of 90 amino acids and a stop codon. Alignment of homologous DNA and amino acid sequences showed that the GnRH of Lasperodomys brandtii belonged to type Ⅰ, and shared the highest identify with Microtus ochrogaste GnRH1. GnRH mRNA was expressed in the hypothalamus, pituitary, testis, adrenal gland, intestine and bladder. The levels of serum testosterone/serum estradiol levels in male/female voles of 8-weeks/36-weeks/80-weeks were significantly higher than those of 4-weeks voles. The expression of GnRH in hypothalamus of 80-weeks voles was in the highest level, while there was no significant difference between 8-weeks/36-weeks voles and 4-weeks voles. We suggested that the possible reason was that the 4-week hypothalamic GnRH was inhibited by the center of the GnRH modulator, while the 8-week/36-week hypothalamic GnRH depended on feedback suppression of steroid. With the increasing age to 80 weeks, sex steroid-mediated feedback suppression declined and response time prolonged, leading to the high GnRH mRNA level in this stage. These results provide basic data for exploring the regulation of reproduction in Brandt’s vole.
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