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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Distribution Regularities of Microsatellites in the Moschus berezovskii Genome
Author of the article:LU Ting, WANG Cheng, DU Chao, LIU Shu, SHENG Yongmei, ZHANG Xiuyue, YUE Bisong
Author's Workplace:1. College of Life Sciences, Sichuan University, Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife; 2. Sichuan medicinal animal Engineering Technology Research Center
Key Words:Moschus berezovskii; genome; microsatellite; distribution regularities
Abstract:The forest musk deer (Moschus berezovskii) is a critically endangered species. Perfect microsatellite number and distribution regularities of microsatellites in the forest musk deer genome were analyzed by microsatellite search tool. A repertoire of 665 524 perfect SSRs with 1–6 bp nucleotide motifs were scanned, accounting for 0.42% of 2.53 Gb forest musk deer genome. The abundance of microsatellites is 244 no. /Mbp in forest musk deer genome. Mono-nucleotide is the most abundant category with the highest relative abundance (81.05 SSRs/Mb), accounting for 33.22 % of all the SSRs, followed by di-nucleotide (21.68 %), penta-nucleotide (21.09 %), tri-nucleotide (18.08 %), and tetra-nucleotide (5.86 %). The ten most abundant microsatellite repeats in forest musk deer genome are A, AACTG, AGC, AC, AT, AG, AAAT, AAC, AAT, and AAAC, accounting 93.2% and shows an apparent A and T preference. The number of microsatellites located on the coding sequence (2 530) is less than that on the non-coding sequence such as introns (200 906) and intergenic regions (454 596), which is consistent with previous studies. This study provides adequate material for the future study of forest musk deer.
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