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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Effects of Copper Stress on Hemocytes Toxicity and caspase-3, α-2M Gene Expression of Macrobrachium rosenbergii
Author of the article:GUO Hui, ZHU Xiaowen, OU Ronghua, LU Zhicheng, TAN Cuiting, SHEN Yuchun, ZHU Chunhua*
Author's Workplace:Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong Province 524025, China
Key Words:Macrobrachium rosenbergii; copper; hemocytes toxicity; gene expression; apoptosis
Abstract:The present study analyzed the toxic effects of Cu on apoptotic cell ratio, ROS production, esterase activities, NO production, and the expression level of caspase-3 and α-2M in Macrobrachium rosenbergii hemocytes. The results showed that the changes of apoptotic cell ratio were consistent with ROS production which was increased significantly (P<0.05) after exposure for 6~48 h. Esterase activities were significantly decreased (P<0.05) at 3~48 h and the minimum was observed at 48 h. The NO production continues to increase and significant differences (P<0.05) were observed after exposure for 6~48 h. The relative expression level of caspase-3 were significantly induced (P<0.05) after exposure for 6~48 h, and reached the peak at 24 h. The relative expression of α-2M was significantly increased (P<0.05) and reached the peak at 12 h, and then significant increase (P<0.05) was also observed although the expression of α-2M temporarily reduced at 24 h. However, α-2M expression level was significantly decreased (P<0.05) at 48 h. Correlation analysis showed that significant and positive correlation (P<0.001) between apoptotic cell ratio and ROS production was observed, as well as apoptotic cell ratio and NO production. All these results suggested that esterase activities were significantly inhibited after Cu exposure while NO, ROS production and α-2M expression level were induced to against Cu exposure. By stress time increasing, overfull production of ROS and NO caused oxidative stress to shrimp and then induced the expression level of caspase-3 which finally resulted in apoptosis, indicating that Cu stress may have immunosuppressive and toxic effects to M. rosenbergii, the increase of NO and ROS production might be the main cause of apoptosis.
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