Comparison of mRNA levels of PPP2CA and PME-1 genes in the testes of yaks and sterile cattle-yaks
Author of the article:REN Liang, FU Wei, JIN Suyu, HUANG Lin, LI Caixia,
Author's Workplace：1 College of Life Science and Technology, Southwest University for Nationalities；2 Jiange Animal Disease Control and Prevention Centre
Key Words： yak; cattle-yak; hybrid male sterility; protein phosphatase 2A; protein methylesterase 1
Abstract：Protein phosphatase 2A (PP2A) participates in the protein reversible phosphorylation, and plays a central role in phosphorylation-dependent signaling pathways. Its activity is regulated by protein methylesterase 1 (PME-1) and other factors. To elucidate the molecular mechanism of hybrid male sterility of yaks, this study cloned cDNA sequences of catalytic subunit α (PPP2CA) of PP2A and PME-1 genes. The coding regions of yak PPP2CA and PME-1 genes are 930 bp and 1143 bp, respectively. The deduced animo acid sequences are conserved. Quantative PCR assay showed that cattle-yaks (n=7) contained dramatically reduced mRNA levels of PPP2CA and PME-1 genes in testes than yaks (n=13, P<0.01). The present results suggest that the reduced mRNA levels of the two genes in the testes might influence some important signaling pathways and associate with hybrid male sterility of cattle-yaks.