Study on chronic toxicity of freeze-dried powdered Cassiae Semen in Sprague-Dawley rats
Author of the article:WEI Ruoyao, PEI Yanyu, SUN Jingjiang, GAO Hong *
Author's Workplace：Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) &amp;amp; Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, PR China
Key Words：Freeze-dried powder Cassiae Semen; Chronic toxicity; NOAEL; The primary toxicity organ
Abstract：Abstract Objective We evaluated the toxicity of freeze-dried powdered Cassiae Semen in male and female Sprague-Dawley rats. Methods Rats were intragastrically administered freeze-dried powdered Cassiae Semen at a dose of 0, 0.5, 2.2, or 10.0 g/kg body weight/day for 26 weeks; several variables were assessed after 13 and 26 weeks as well as after a 4-week recovery period. Result No mortality was observed in the treated animals, and body weight increased in the animals in a dose-dependent manner. In males, at 26 and 39 weeks, there were significant increases in relative
kidney weights in the 2.2 and 10.0 g/kg groups compared with that in the 0 g/kg group. Pigment deposition in the epithelial cells of the renal proximal convoluted tubules and atrophy or regeneration of renal tubules were observed in the 10.0 g/kg group after 52 weeks, and these changes were not fully reversed after the 4-week recovery period. Conclusion Under the studied conditions, the No Observed Adverse Effect Level (NOAEL) for freeze-dried powdered Cassiae Semen was 2.2 g/kg in rats, and the primary toxicity organs in the 10.0 g/kg group were the kidneys.