Anti-fertility Effect of Peppermint Oil on Female Lasiopodomys brandtii
Author of the article:ZHANG Xingli, XIE Chenwen, LIU Xiaoxue, FANG Wei,
Author's Workplace：college of life science, sichuan university, chengdu 610064, china
Key Words：Lasiopodomys brandtii; peppermint oil; embryo implantation; anti-fertility.
Abstract：The occurrence area of grassland rodents in China has been increasing year by year, Brandt’s vole (Lasiopodomys brandtii) is a major rodent grassland pest found in Inner Mongolia, mainly inhabiting degraded pasture, and rapidly spreading due to its high breeding rate. Aiming to find a more effective way to control the number of rodents, the anti-fertility effect of peppermint oil on the Brandt's voles was investigated. The mature non-overwintering Brandt's voles were mated in a 2:1 ratio (female:male). Then the pregnant voles were randomly divided into 3 treatment groups (the concentration of peppermint oil: 750 mg·kg-1, 1 000 mg·kg-1, 1 500 mg·kg-1) and 1 control group. After intragastric administration for 7 days, the voles were gavaged. The number of the implanted uterus was counted, and the histopathological study of uterus was carried out. The results showed that peppermint oil could reduce the number of implantation embryos in Brandt’s voles. The scanning electron microscope results showed that the endometrial receptivity of Brandt's voles administrated by peppermint oil was changed. Histopathological results showed that the uterus morphology of Brandt's voles treated with peppermint oil was also changed and the immunohistochemical results showed that the expression levels of estrogen receptor α (ERα), progesterone receptor (PR), E-cadherin, and matrix metalloproteinase-2 (MMP2) were all abnormal. These studies showed that the anti-fertility effect of peppermint oil on Brandt's voles was mainly through regulating the expression level of ER, PR, MMP2 and E-cadherin to affect endometrial receptivity and embryo implantation, which led to implantation failure. Therefore, the peppermint oil can be used as a potential plant-derived infertility in rodent sterility control.