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Baff转基因斑马鱼的构建及相关基因表达检测
The Construction of Transgenic Zebrafish Overexpressing Baff and the Detection of baff-related Genes Expression
张力1,谢英1,周昕2,徐增年1,刘树锋1*
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DOI:
作者单位: (1. 河北医科大学实验动物学部,河北省实验动物实验室,石家庄050017;2. 河北医科大学第三医院检验科,石家庄050051)
中文关键字:斑马鱼;红斑狼疮;baff;bcl-2; il-4
英文关键字:zebrafish; systemic lupuserythematosus; baff; bcl-2; il-4
英文摘要: baff full length cDNA was cloned by RT-PCR and constructed to overexpression plasmid pIRES2-GFP. The baff overexpression zebrafish were successfully obtained by microinjection and fluorescence screening. The expression levels of baff, GFP as well as baff downstream regulated genes bcl-2 and il-4 in transgenic embryos were determined by qPCR. The western blot result confirmed the expression of Baff-GFP fused protein. The result of qPCR demonstrated the overexpression of GFP and baff in transgenic embryos and juvenile fish. However, although the expression of baff downstream regulated gene bcl-2 was significantly increased, the il-4 gene showed a impaired expression. In conclusion, the transgenic zebrafish overexpressing baff can be obtained by embryo microinjection, and this is a fundamental work for the establishment of lupus erythematosus transgenic zebrafish model and high throughput screening for Baff antagonist.
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国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
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