The Genetic Variation Analysis of Microsatellite among the Gili Tilapia (Oreochromis niloticus ♀×Sarotherodon melanotheron♂) and Its Parents
英文关键字：Gili tilapia; Sarotherodon melanotheron; Oreochromis niloticus; microsatellite; genetic diversity
英文摘要：Gili tilapia, a F2 hybrid offspring by Oreochromis niloticusb (♀)×Sarotherodon melanotheron (♂), had been examined and approved by the China National Aquaculture Variety Approval Committee as a new variety for aquaculture in 2009. S. melanotheron showed stronger salt-tolerance while O. niloticus presented faster growth rate. Six pairs of microsatellite primers were chosen to analyze the genetic characteristics of Gili tilapia and its parents. The results were shown as follows: (1) The sizes of the product amplified by the six microsatellite primer pairs was ranged from 180 to 350 bp. A total of 21 alleles were found and significant difference was observed among the groups, microsatellite loci and alleles; (2) The value of effective allele number (Ne), Nei's gene diversity index (H) and polymorphic information content rankings (PIC) were Gili tilapia>O. niloticus>S. melanotheron. Gili tilapia showed a highly polymorphic and the PIC value was 0.657. (3) The genetic distance between Gili tilapia and S. melanotheron was closer than that of Gili tilapia and O. niloticus, which showed S. melanotheron had more important influence on Gili tilapia than O. niloticus.