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黑斑蛙重盘吸虫的线粒体基因组特征及其系统发育研究
Mitochondrial Genome Characterization and Phylogeny of Diplodiscus nigromaculati
李宗宪1, 李寄仟1, 徐伟江1,2, 范丽仙1,2*
点击:162次 下载:22次
DOI:10.11984/j.issn.1000-7083.20210372
作者单位:1. 云南师范大学生命科学学院, 昆明 650500;
2. 生物能源持续开发利用教育部工程研究中心, 昆明 650500
中文关键字:黑斑蛙重盘吸虫;同盘总科;重盘科;线粒体基因组;宿主新记录
英文关键字:Diplodiscus nigromaculati; Paramphistomoidea; Diplodiscidae; mitochondrial genome; new host record
中文摘要:重盘科Diplodiscidae Cohn, 1904复殖吸虫是两栖动物最为常见的寄生虫,形态多样,目前未见基于线粒体基因组的遗传多样性研究。本研究记述并分析了寄生于滇蛙Dianrana pleuraden肠道的重盘科重盘属Diplodiscus Diesing, 1836黑斑蛙重盘吸虫D. nigromaculati的线粒体全基因组序列,为后续开展此类吸虫的遗传结构及遗传多样性研究提供了新的数据。黑斑蛙重盘吸虫线粒体基因组全长14 697 bp(GenBank登录号:MW698822),由22个tRNA基因、12个蛋白编码基因、2个rRNA基因和1个非编码区组成;AT含量(60%)高于GC含量(40%),但AT含量为目前已报道同盘总科Paramphistomoidea Fischoeder, 1901中最低;trnG基因和trnE基因的互换成为同盘总科吸虫线粒体基因组遗传结构的重要特征。基于12个蛋白编码基因的系统发育树支持重盘属隶属于重盘科。本研究中,滇蛙为黑斑蛙重盘吸虫的宿主新记录。
英文摘要:Trematodes of family Diplodiscidae Cohn, 1904 are common parasites of amphibian, whose morphological diversities have widely been reported. However, the research on the genetic diversities based on mitochondrial genome is still undocumented. The complete mitochondrial genome of Diplodiscus nigromaculati (Diplodiscus Diesing, 1836) from the intestinal tract of host Dianrana pleuraden was described and analyzed in the present study. The complete mitochondrial genome of D. nigromaculati is 14697bp long (GenBank accession number:MW698822), which is composed of 22 tRNA genes, 12 protein-coding genes, 2 rRNA genes and 1 noncoding region. The content of AT (60%) in the mitochondrial genome is higher than that of GC (40%), which is the lowest in reported species of Paramphistomoidea Fischoeder, 1901. The exchange of trnG and trnE indicate an important genetic structure feature of mitochondrial genome of flukes of the Paramphistomoidea. The phylogeny analysis using concatenated amino acid sequences for 12 protein-coding genes supported that Diplodiscus belongs to Diplodiscidae. This study indicated that D. pleuraden is the new host record of D. nigromaculati. These results provided basic data and reference for the future research of genetic structure and genetic diversity of this group of fluke.
2022,41(3): 248-255 收稿日期:2021-11-01
分类号:Q959.155
基金项目:国家自然科学基金项目(31560589;32060115;30660022;30960049;31260507)
作者简介:李宗宪(1996—),男,硕士研究生,主要从事鱼类两栖类复殖吸虫分类学研究,E-mail:zongxian_li514@126.com
*通信作者:范丽仙,主要从事寄生虫分类学研究,E-mail:anne330@163.com
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