刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610065
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2020年第39卷第6期

中华蟾蜍多组织差异转录组分析
Differential Transcriptome Analysis in Multi-Tissue of Bufo gargarizans
胥文才1, 蔺杼阳1, 肖雅丹1, 唐瑞祥1, 范振鑫1,2, 孟杨1,2*
点击:229次 下载:24次
DOI:10.11984/j.issn.1000-7083.20200278
作者单位:1. 四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 成都 610065;
2. 四川大学生命科学学院, 四川省濒危动物保护生物学重点实验室, 成都 610065
中文关键字:中华蟾蜍;转录组;耳后腺;RNA-Seq
英文关键字:Bufo gargarizans; transcriptome; retroauricular gland; RNA-Seq
中文摘要:中华蟾蜍Bufo gargarizans的皮肤产物蟾酥及其褪皮而得的蟾衣具有极高的药用价值,现代医学研究鉴定并证明了蟾酥和蟾衣内含有多种有效的生物成分。本研究使用RNA-Seq对1只中华蟾蜍的肝脏、脾脏、心脏、卵泡、肌肉、腹部皮肤、右耳后腺共7个组织的转录组进行测序并分析,使用de novo完成了其多组织转录本的组装构建,得到了具有较高代表性的转录组,并在各大蛋白质数据库进行同源性比对,完成了中华蟾蜍多组织的转录组功能注释。计算各组织转录本的表达量,比较表达量鉴定出耳后腺与其他组织间共计3 668个显著上调基因和260个显著下调基因。上述差异表达基因显著富集到了蛋白质合成、酶的调节、分子跨膜转运体生成和前体物质的代谢合成通路上,提示中华蟾蜍耳后腺相对于其他组织在相应甾族化合物的合成代谢上具有显著优势。本研究提供了中华蟾蜍多组织转录组数据,为今后深入其基因表达提供了支持,并且对蟾酥分泌等的进一步研究提供了转录组学参考,有助于完善蟾酥、蟾衣的药用研究。
英文摘要:Venenum bufonis, the skin products of Chinese toad (Bufo gargarizans) and toad-cortex, its sloughed skin has an extremely high medicinal value. Modern medical research has identified and proved that they contain a large variety of effective biological components. In our research, RNA-Seq is sequenced to analyze the transcriptome of a toad's 7 tissues in total, including liver, spleen, heart, follicles, muscles, abdominal skin and retroauricular gland of the right ear. Next the de novo method is used to complete the assembly and construction of multi-tissue transcripts of B. gargarizans, thus a highly representative transcriptome is obtained. Then by performing homology comparisons among major protein databases, the transcriptome functional annotation of the multi-tissues in B. gargarizans is completed. By calculating and comparing the expression levels of transcripts in various tissues, a total of 3 668 significantly up-regulated genes and 260 significantly down-regulated genes are obtained between the retroauricular gland and other tissues of B. gargarizans. The functional enrichment analysis of the above-mentioned differentially expressed genes find that they are significantly enriched in protein synthesis, enzyme regulation, molecular transmembrane transporter generation, and metabolic synthesis pathways of precursor substances, suggesting that there is a significant advantage in the synthesis and metabolism of corresponding steroids in the retroauricular gland of B. gargarizans. These data in this study provide a multi-tissue transcriptome of B. gargarizans, and provide support for more in-depth studies on the gene expression of B. gargarizans in the future. They also provide a valuable resource for further research on the toad secretions of B. gargarizans, which can help to improve the research on the medicinal value of venenum bufonis and toad-cortex.
2020,39(6): 601-615 收稿日期:2020-07-22
分类号:Q959.5;Q811.4
基金项目:好医生药业集团助研项目
作者简介:胥文才(1999-),本科生,主要研究领域为生物信息学研究,E-mail:604295323@qq.com
*通信作者:孟杨,E-mail:mengyang@scu.edu.cn
参考文献:
陈丽萍, 何丽莉, 巴云飞. 2011. 中华大蟾蜍抗菌肽对癌细胞的生长抑制作用[J]. 中国现代医生, 49(36): 24-27.
杜联明, 李午佼, 沈福军, 等. 2014. 大熊猫血液转录组分析[C]. 青海省动物学会. 第三届中国西部动物学学术研讨会论文摘要集.
国家药典委员会. 2010. 中华人民共和国药典 一部[M]. 北京: 中国医药科技出版社.
晋家正, 李午佼, 牟必琴, 等. 2018.药用美洲大蠊全基因组测序分析[J].四川动物, 37(2): 121-126.
罗展雄, 倪秉强, 郑青平, 等. 2009. 华蟾素注射液对晚期恶性肿瘤患者T淋巴细胞亚群及NK细胞的影响[J]. 现代肿瘤医学, 17(2): 340-341.
彭贝, 巩仔鹏, 陈涛. 2011. 华蟾素注射液治疗肝癌的基础和临床研究进展[J]. 药物评价研究, 35(15): 2453-2455.
祁云霞, 刘永斌, 荣威恒. 2011. 转录组研究新技术: RNA-Seq及其应用[J]. 遗传, 33(11): 1191-1202.
孙崇峰, 范圣此, 罗毅, 等. 2018. 蟾酥化学成分及其人工合成的研究进展[J]. 中草药, 49(13): 3183-3192.
王斌, 李德远. 2009. 细胞色素P450的结构与催化机理[J]. 有机化学, 29(4): 658-662.
吴文英, 李丕鹏, 陆宇燕. 2011. 花背蟾蜍和中华蟾蜍皮肤腺体及耳后腺体的组织学研究[J]. 蛇志, 23(1): 20-25.
辛秀兰, 张宝璟, 苏东海, 等. 2012. 中药蟾酥的药理作用研究进展[J]. 现代生物医学进展, 12(3): 588-600.
杨宏梅, 陈涛. 2014. 华蟾素在消化系统肿瘤治疗中应用的研究进展[J]. 广东医学, 34(1): 63-66.
张立, 普布次仁, 胡亚东, 等. 2020. 产双羔彭波半细毛羊发情期卵巢基因表达差异研究[J]. 四川动物, 39(2): 156-166.
赵大洲, 陈继永, 秦勇, 等. 2006. 中华大蟾蜍蟾酥与蟾皮化学成分的比较分析[J]. 天津药学, 18(4): 21-24.
Altschul SF, Gish W, Miller W, et al.1990. Basic local alignment search tool[J]. Journal of Molecular Biology, 215(3): 403-410.
Chao W, Zhang YH, Chai LH, et al. 2018. Transcriptomics provides mechanistic indicators of fluoride toxicology on endochondral ossification in the hind limb of Bufo gargarizans[J]. Aquatic Toxicology, 201: 138-150.
Conesa A, Götz S, García-Gómez JM, et al. 2005. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research[J]. Bioinformatics, 21(18): 3674-3676.
Davidson NM, Oshlack A. 2014. Corset: enabling differential gene expression analysis for de novo assembled transcriptomes[J]. Genome Biology, 15(7): 410.
Grabherr MG, Haas BJ, Yassour M, et al. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 29(7): 644-652.
Haas BJ, Papanicolaou A, Yassour M, et al. 2013. De novo transcript sequence reconstruction from RNA-Seq using Trinity platform for reference generation and analysis[J]. Nature Protrol, 8(8): 1494-1512.
Kim D, Paggi JM, Park C, et al. 2019. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype[J]. Nature Biotechnology, 37(8): 907-915.
Meyer UA.1996. Overview of enzymes of drug metabolism[J]. Journal of Pharmacokinetics and Biopharmaceutics, 24(5): 449-459.
Moriya Y, Itoh M, Okuda S, et al. 2007. KAAS: an automatic genome annotation and pathway reconstruction server[J]. Nucleic Acids Research, 35(2): 182-185.
Pruitt KD, Tatusova T, Maglott DR. 2005. NCBI reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins[J]. Nucleic Acids Research, 33(1): 501-504.
Robinson MD, McCarthy DJ, Smyth GK. 2010. edgeR: a bioconductor package for differential expression analysis of digital gene expression data[J]. Bioinformatics, 26(1): 139-140.
Simão FA, Waterhouse RM, Ioannidis P, et al. 2015. BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs[J]. Bioinformatics, 31(19): 3210-3212.
Xie C, Mao X, Huang Y, et al. 2011. KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases[J]. Nucleic Acids Research, 39(2): 316-322.
Yang WZ, Qi Y, Lu B, et al. 2017. Gene expression variations in high-altitude adaptation: a case study of the Asiatic toad (Bufo gargarizans)[J/OL]. BMC Genetics, 18(1): 62[2020-05-30]. https://doi.org/10.1186/s12863-017-059-z.
Ye J, Fang L, Zheng H, et al. 2006. WEGO: a web tool for plotting GO annotations[J]. Nucleic Acids Research, 34(2): 293-297.
Yu G, Wang L, Han Y, et al. 2012. clusterProfiler: an R package for comparing biological themes among gene clusters[J]. OMICS: A Journal of Integrative Biology, 16(5): 284-287.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2021四川动物》编辑部 蜀ICP备08107403号-3
您是本站第9419034名访问者

川公网安备 51010702000173号