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基于COⅠ基因探讨北京及其周边地区跳钩虾种群遗传结构
Population Genetic Structure of Platorchestia japonica From Beijing and Its Adjacent Regions Based on COⅠ Gene Sequences
崔昕宇1, 刘宏广2, 李枢强2*
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DOI:10.11984/j.issn.1000-7083.20180289
作者单位:1. 中国人民大学附属中学, 北京 100080;
2. 中国科学院动物进化与系统学重点实验室, 中国科学院动物研究所, 北京 100101
中文关键字:跳钩虾;COⅠ;系统发育;遗传结构;遗传多样性
英文关键字:Platorchestia japonica; COⅠ; phylogeny; genetic structure; genetic diversity
中文摘要:跳钩虾Platorchestia japonica栖息于湖泊、河流岸边,是重要的环境指示生物。本研究以线粒体COⅠ基因片段为分子标记,对北京及其周边地区22个采样点的128个样本进行种群遗传多样性和遗传结构研究。结果显示,623 bp的COⅠ基因序列中有567个保守位点、56个变异位点和38个简约信息位点。128个样本检测到43个单倍型,单倍型多样性为0.938,核苷酸多样性为0.011 72。最大似然法和贝叶斯法构建的系统发育树以及单倍型网络图表明,研究区域跳钩虾没有明显的地理种群结构,但所有单倍型形成2个遗传进化支。分子变异分析结果证实,跳钩虾2个进化支间的遗传变异显著高于进化支内的,进化支间固定系数为0.852 19,表明2个进化支遗传分化明显。本研究为进一步研究中国区域跳钩虾的遗传结构提供了有意义的基础数据。
英文摘要:Crustaceans of Platorchestia japonica have been investigated for their suitability to serve as indicators for the quality of water bodies, since they normally live along rivers and lakes. This study intends to explore the population genetics of P. japonica around Beijing and its adjacent regions. The mitochondrial COⅠ genes of 128 P. japonica individuals collected from 22 sampling sites were sequenced. Polymorphism analysis of P. japonica COⅠ gene showed that there were 567 conserved sites, 56 variable sites, and 38 parsimony informative sites. Genetic diversity analysis discovered 43 haplotypes, with haplotype diversity of 0.938 and nucleotide diversity of 0.011 72. Phylogenetic analyses using Maximum Likelihood, Bayesian methods and network analyses revealed that the haplotypes of P. japonica showed no apparent phylogeographic structure, but all haplotypes clustered into 2 clades. The results of molecular variance analyses approved that the genetic variation among the clades of P. japonica was significantly higher than that within clades, with a Fst value of 0.852 19. These findings provide a scientific basis for further studying the genetic structure of P. japonica in China.
2019,38(4): 402-407 收稿日期:2018-09-29
分类号:Q173;Q953
基金项目:国家自然科学基金项目(31772417)
作者简介:崔昕宇(2003-),女,高中生
*通信作者:李枢强,E-mail:lisq@ioz.ac.cn
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