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家鸡钠尿肽受体B基因的克隆和组织表达分析
Molecular Cloning and Tissue Expression of Chicken Natriuretic Peptide Receptors B Gene
李正阳, 张剑南, 陈军安, 李娟, 王亚军*
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DOI:10.11984/j.issn.1000-7083.20180144
作者单位:四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 成都 610065
中文关键字:NPR-B;基因克隆;功能分析;组织表达;生理功能
英文关键字:NPR-B; gene cloning; functional analysis; tissue expression; physiological function
中文摘要:钠尿肽受体B(NPR-B)作为C-型钠尿肽(CNP)的选择性受体,可介导CNP的多种生理效应,包括调控垂体激素释放、维持心血管系统稳定、调节神经系统发育、调控细胞增殖及促进骨骼生长等。本研究以家鸡Gallus gallus domesticus为模型,首次报道了NPR-B基因的全长cDNA,并检测其在成体家鸡中的组织表达图谱。结果显示:家鸡NPR-B基因cDNA全长为3 189 bp,可编码1 062个氨基酸。家鸡NPR-B与人Homo sapiens、大鼠Rattus norvegicus、非洲爪蟾Xenopus laevis和斑马鱼Danio rerio分别具有79%、78%、73%、67%的氨基酸序列一致性;同时采用实时荧光定量PCR技术探究家鸡NPR-B基因的组织表达分布,发现其在心脏、肌肉、中脑和垂体(头部)中表达水平较高。本研究结果为阐释CNP及其选择性受体NPR-B在家鸡中的生理功能奠定基础。
英文摘要:As a selective receptor of C-type natriuretic peptide (CNP), the natriuretic peptide receptor B (NPR-B) regulates various physiological processes, including pituitary hormone secretion, cardiovascular stability, nervous system development, cell proliferation and bone growth. In this study, the full-length cDNA of Gallus gallus domesticus NPR-B gene was reported and the tissue expression of NPR-B gene in adult chickens was detected by quantitative real-time PCR (qPCR). The results showed that the full length of chicken NPR-B gene was 3 189 bp encoding a protein of 1 062 amino acids. The amino acid sequence of NPR-B was highly homologous to that of Homo sapiens (79%), Rattus norvegicus (78%), Xenopus laevis (73%) and Danio rerio (67%). The result of qPCR showed that NPR-B gene was highly expressed in the heart, muscle, midbrain and pituitary of chicken. These findings laid a foundation for further explaining the physiological functions of CNP and NPR-B in chickens.
2018,37(6): 653-659 收稿日期:2018-05-03
分类号:Q959.7;Q78
基金项目:国家自然科学基金项目(31771375)
作者简介:李正阳(1991—),硕士,主要从事动物分子遗传学研究,E-mail:lzy1991716@outlook.com
*通信作者:王亚军,E-mail:cdwyjhk@gmail.com
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