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        CN 51-1193/Q


Synergistic Effect of Recombinant Yeast Co-Expressing Porcine Interleukin-2 and Fusion Antimicrobial Peptide Gene on Immunity and Growth of Mice
胡冰1#, 吴雪颖1#, 马常俊1#, 万小平1, 肖永乐1, 陈建林1, 李江淩2, 吕学斌2*, 王泽洲3*, 高荣1*
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作者单位:1. 四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 四川省动物疫病预防与食品安全重点实验室, 成都 610065;
2. 四川省畜牧科学研究院, 成都 610066;
3. 四川省动物疫病控制中心, 成都 610035
英文关键字:porcine interleukin-2; fusion antimicrobial peptide; recombinant Pichiapastoris; mice; immunity
英文摘要:To develop a novel and practicable immunomodulator, the recombinant Pichiapastoris to co-express porcine interleukin-2 and fusion antimicrobial peptide gene were constructed by 2A self-cleavage technique, and then fermented to feed ICR mice by gavage to evaluate its effects on the immunity and growth of mice via real time fluorescent quantitative PCR, ELISA and flow-cytometry. It was found that the expressed molecules of recombinant yeasts not only showed remarkable immunological bioactivity but also manifested obvious antimicrobial activity in vitro. The feeding of the yeast relatively improved the growth of mice in comparison to the control group (P<0.05). The contents of IgG, IgG1 and IgG2a were significantly increased in the yeast-treated groups (P<0.05), and the expression levels of the TLR1, TLR4, TLR6, TLR9, IL-7, IL-15, IL-23, CD62L, IL-2, IL-4, IL-6, IL-12, CRP4 and CAMP genes significantly increased in comparison to the control (P<0.05). Similarly, significant increases of the leukocytes, Th and Tc cells were detected in the blood of yeast-treated mice (P<0.05). Meanwhile, the mice of yeast-treated groups displayed markedly higher levels of immunity and survival rates than those of the control after challenged with virulent bacteria Escherichia coli and Staphylococcus aureus (P<0.05). These results suggest that the recombinant yeast can effectively enhance the immunity and growth of mice, and thus can be further developed as a promising safe and effective immunomodulator for livestock.
2018,37(6): 609-619 收稿日期:2017-11-16
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