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乌金猪、青峪猪和成华猪免疫器官与组织Toll样受体和抗菌肽基因表达比较研究
Comparison of Gene Expression of Toll-Like Receptors and Antimicrobial Peptides in the Immune Organs and Tissues of Wujin Pig, Qingyu Pig and Chenghua Pig
张敬梅1#, 顾以韧2#, 李江淩2, 陈晓晖2, 曾凯2, 吕学斌2*, 高荣1*
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作者单位:1. 四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 动物疫病防控和食品安全四川省重点实验室, 成都 610065;
2. 四川省畜牧科学研究院, 成都 610066
中文关键字:猪;Toll样受体基因;抗菌肽基因;基因表达;免疫器官和组织
英文关键字:pig; toll-like receptors gene; antimicrobial peptides; gene expression; immune organs and tissues
中文摘要:Toll样受体(TLRs)和抗菌肽是机体天然免疫系统的重要组成部分,是抵御外界有害微生物或病毒入侵的重要屏障。乌金猪、青峪猪、成华猪是中国优良地方猪种,具有繁殖力高、抗病耐逆性强等优点。本实验比较了8月龄乌金猪、青峪猪、成华猪与约克夏猪的消化道、呼吸道局部免疫组织和全身免疫器官的先天免疫特点,采用实时荧光定量PCR技术检测TLRs(TLR1、TLR2、TLR4、TLR7、TLR9)和抗菌肽(pBD-1、PR-39)基因在4种猪的胸腺、脾脏、扁桃体、肠系膜淋巴结和肺门淋巴结中的表达差异。结果显示,成华猪胸腺中TLR7、PR-39,脾脏中TLR4、TLR7,肠系膜淋巴结中TLR4、TLR9,肺门淋巴结中TLR1、TLR4、TLR7、TLR9 mRNA表达水平显著高于其他猪种(P<0.05);乌金猪胸腺中TLR1、TLR9,扁桃体中TLR1、TLR2、TLR4、TLR7、TLR9、pBD-1,肠系膜淋巴结中TLR7 mRNA表达水平显著高于其他猪种(P<0.05);青峪猪扁桃体和肠系膜淋巴结中PR-39 mRNA表达水平显著高于其他猪种(P<0.05)。这些结果为进一步利用优良地方猪种杂交育种和筛选抗病分子标记提供了新的线索。
英文摘要:Toll-like receptors (TLRs) and antimicrobial peptides are important components of the host natural immune system and are an important barrier to prevent harmful microorganisms or viruses. Wujin pig, Qingyu pig and Chenghua pig are the well-known Chinese unique local subspecies of pig breed, and they own the merits of high fertility and strong disease resistance. In this study, the innate immunity characteristics of 8-month-old Wujin pigs, Qingyu pigs, Chenghua pigs and Yorkshire pigs were compared by using the local immune tissues and systemic immune organs. Real-time fluorescent quantitative PCR was used to detect the expression levels of TLRs (TLR1, TLR2, TLR4, TLR7 and TLR9) and antimicrobial peptide-encoding genes (pBD-1 and PR-39) in thymus, spleen, amygdala, mesenteric lymph node and hilar lymph nodes. The results showed that compared to the control pigs, the expression levels of TLR7 and PR-39 in the thymus, TLR4 and TLR7 in the spleen, TLR4 and TLR9 in the mesenteric lymph nodes, and TLR1, TLR4, TLR7 and TLR9 in the hilar lymph nodes were significantly higher (P<0.05) in Chenghua pigs; the expression levels of TLR1 and TLR9 in the thymus, TLR1, TLR2, TLR4, TLR7, TLR9 and pBD-1 in the tonsils, and TLR7 in the mesenteric lymph nodes of Wujin pig were significantly higher than those in other pig breeds (P<0.05); the expression of PR-39 gene in the tonsils and mesenteric lymph nodes of Qingyu pigs was significantly higher than those in other pig breeds (P<0.05). These results provide useful clues for further screening the disease-resistant molecular markers of local pig hybrid breeding.
2018,37(5): 525-532 收稿日期:2018-03-13
DOI:10.11984/j.issn.1000-7083.20180086
分类号:S852.4;Q344+.13
基金项目:成都市科技攻关项目(2015NY-02-0028-NC);四川省国际科技合作项目(2017HH0023);四川省科技支撑项目(2016NYZ0042,2016RZ0034)
作者简介:张敬梅(1991-),女,硕士研究生,研究方向:分子遗传学与基因工程,E-mail:1309458653@qq.com;顾以韧(1980-),男,副研究员,博士研究生,研究方向:猪遗传育种,E-mail:guyiren1128@163.com
*通讯作者:吕学斌,E-mail:lake96@qq.com;高荣,E-mail:gaorong96@163.com
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