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白腹鹭COⅠCyt b基因序列分析及鹭属系统发育研究
Gene Sequences of COⅠ and Cyt b of Ardea insignis and Molecular Phylogenetic Analysis of Genus Ardea
陈熙1, 王斌2, 罗旭1*
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作者单位:1. 西南林业大学, 西南地区生物多样性保育国家林业局重点实验室, 昆明 650224;
2. 云南高黎贡山国家级自然保护区泸水管护分局, 云南泸水 673100
中文关键字:白腹鹭;COⅠ基因;Cyt b基因;系统发育树
英文关键字:Ardea insignis; COⅠ gene; Cyt b gene; phylogenetic tree
中文摘要:白腹鹭Ardea insignis数量稀少,被世界自然保护联盟列为极危(CR)物种,关于该物种的生态信息和分子生物学研究极少。本研究通过扩增得到了白腹鹭的2个线粒体基因序列(COⅠCyt b),Cyt b基因序列长度为1 163 bp,其中的A+T含量(51.7%)高于C+G(48.3%),第三位点上碱基偏倚最突出;COⅠ基因序列长度为722 bp,A+T含量(52.5%)高于C+G(47.5%),碱基偏倚同样在第三位点最明显。结合来自GenBank鹭属Ardea其他6个物种的COⅠCyt b基因序列,构建鹭属的系统发育树,结果表明,鹭属7种鸟类构成单系,后验概率支持度为96%,大白鹭A. alba和白腹鹭在鹭属中是较早分化出的类群,位于鹭属分支的基部;白腹鹭与草鹭A. purpurea的遗传距离最近。
英文摘要:Ardea insignis is a rare species and listed as a critically endangered species in the IUCN red list of threatened species. It belongs to genus Ardea of family Ardeidae. There are very few studies carried on this species. We obtained COⅠ and Cyt b gene sequences of A. insignis using PCR technology. The length of the Cyt b gene was 1 163 bp, and the A+T content (51.7%) was higher than that of C+G (48.3%). The length of the COⅠ gene was 722 bp, and the A+T content (52.5%) was also higher than that of C+G (47.5%). The third site of both genes showed the most remarkable base bias. Combined with the sequences of the other 6 Ardea species from GenBank, the phylogenetic tree of genus Ardea was constructed. The result showed that 7 species of genus Ardea formed a monophyletic clade with the posterior support at 96%. A. alba and A. insignis were found at the basal position of this clade, indicating their early diverge. Based on the genetic analysis, A. purpurea was considered to be the most closely related species to A. insignis.
2018,37(3): 268-273 收稿日期:2017-11-10
DOI:10.11984/j.issn.1000-7083.20170353
分类号:Q959.7
基金项目:环保部生物多样性示范观测项目(2016-2017);云南省极小种群物种拯救保护项目(2016)
作者简介:陈熙(1993-),女,硕士研究生,研究方向:野生动植物保护与利用,E-mail:18387158232@163.com
*通讯作者:罗旭,副教授,研究方向:鸟类进化、生态与保护,E-mail:xu_luo@aliyun.com
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