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大口黑鲈精子超微结构研究
Studies on the Micro-ultrastructure of Micropterus salmoides Sperm
乔志刚, 张晓光, 沈方方, 刘淑琰, 贾武
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作者单位:河南师范大学水产学院, 河南新乡 453007
中文关键字:大口黑鲈;精子;超微结构
英文关键字:Micropterus salmoides; sperm; ultrastructure
中文摘要:为了解大口黑鲈Micropterus salmoides精子的超微结构,应用扫描电镜和透射电镜对大口黑鲈精子结构进行观察。结果显示,大口黑鲈精子由头部、中段和鞭毛三部分组成,扫描电镜下精子中段不明显,无顶体;精子全长25.07 μm±4.93 μm(n=30),头部近球形,直径1.73 μm±0.29 μm(n=30),鞭毛长23.00 μm±4.86 μm(n=30)。头部主要由细胞核构成,细胞核呈蘑菇形,染色质电子致密成簇,被电子透明区分开,核近鞭毛端向内凹陷,形成较浅的核窝。中段包括中心粒复合体和袖套,中心粒复合体由近端中心粒和远端中心粒构成,近端中心粒位于核窝内,与细胞核横轴平行,远端中心粒为鞭毛的基部,位于核窝外,袖套内,与近端中心粒垂直,呈"T"字形。线粒体分布在袖套两侧的袖套腔中,形状大小不一,总数(17±4)个(n=30)。鞭毛从袖套腔中伸出,主要由轴丝和侧鳍构成,轴丝与远端中心粒相接,有典型的"9+2"二联微管结构,侧鳍分布在鞭毛两侧。研究表明,大口黑鲈精子为硬骨鱼类Ⅰ型精子,其袖套形状以及线粒体的数目和大小与鲈形目Perciformes其他鱼类的精子结构存在区别。
英文摘要:The current study investigated the ultrastructure of largemouth bass (Micropterus salmoides) sperm using scanning electron microscope and transmission electron microscope. The sperm of M. salmoides was composed of three major parts:a nearly spherical head without an acrosome, a unconspicuous midpiece, and a slender flagellum. The full-length of sperm is 25.07 μm±4.93 μm (n=30) with a spherical head length of 1.73 μm±0.29 μm in diameter (n=30) and a slender flagellum length of 23.00 μm±4.86 μm long (n=30). The head of sperm is mainly composed of a mushroom-shaped nuclei, and the clustering electron-dense is separated by an electronlucent area. The nucleus near the flagellum segment is a depression, and it forms shallow nuclear fossa. The midpiece of sperm contains the centriolar complex and the sleeve. The centriolar complex is composed of the proximal and distal centriole. The proximal centriole is located inside the nuclear fossa and parallel to the cross axis of nuclei. The ‘T’ shape distal centriole locates outside the nuclear fossa and inside the sleeve is the base of flagellum, and it is perpendicular to the proximal centriole. There are 17±4 unequally sized mitochondria (n=30) inside the central space of both sides of sleeve. The flagellum of sperm stretched out of the central space of sleeve is mainly composed of the axoneme and the lateral fin. The axoneme comprised microtubule configurations in a ‘9+2’ microtubule pattern. The lateral fins are distributed on both sides of flagella. These results revealed that the sperm of M. salmoides should be categorized as Type I, which is clearly distinct from the sperm of other species from order Perciformes in the shape of sleeve, the number and shape of mitochondria. The sleeve structure of M. salmoides is accord with the typical sleeve structure of teleost sperm, but the shape and the number of mitochondria of M. salmoides are similar with amphibian species, indicating that M. salmoides is more original in phylogenetic development than other species of Perciformes.
2017,36(6): 686-691 收稿日期:2017-06-27
DOI:10.11984/j.issn.1000-7083.20170205
分类号:Q952.4;Q959.4
基金项目:河南省高等学校重点科研项目(17B240003);淡水生态与生物技术国家重点实验室开放课题(2017FB08)
作者简介:乔志刚(1965-),博士,教授,从事水产苗种工程及集约化健康养殖研究,E-mail:hnsddsyy@126.com
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