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家鸡G蛋白偶联受体161的基因克隆、分子进化和组织表达
Gene Cloning, Molecular Evolution and Tissue Expression of G Protein-coupled Receptor 161 in Chicken
祝国强, 莫春横, 李正阳, 王亚军, 李娟*
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作者单位:四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 成都 610065
中文关键字:家鸡;GPR161;基因克隆;分子进化;组织表达
英文关键字:chicken; GPR161; gene cloning; molecular evolution; tissue expression
中文摘要:G蛋白偶联受体161(GPR161)是G蛋白偶联受体家族孤儿受体家族成员,在哺乳动物晶状体发育调节和神经胚形成中具有重要作用。近年来研究发现该蛋白罕见地拥有类似支架蛋白的结构特征,暗示其信号转导机制不同于其他G蛋白偶联受体。本研究以家鸡Gallus gallus为动物模型,探究GPR161基因序列信息、分子遗传进化关系以及其组织表达图谱。家鸡GPR161基因编码区序列全长1 566 bp,编码具521个氨基酸的前体蛋白。序列分析显示,家鸡GPR161基因编码区与人Homo sapiens、小鼠Mus musculus、斑马鱼Danio rerio的氨基酸相似度分别为83.0%、82.6%、65.8%。分子进化遗传分析结果显示,GPR161基因在家鸡与斑马鱼的进化关系比家鸡与人或小鼠都更为疏远。利用荧光定量PCR探究家鸡GPR161基因在各组织的表达分布,结果显示,家鸡GPR161基因mRNA在精巢或卵巢、大脑、心脏、肌肉中有较高表达。本研究是鸟类中关于GPR161基因的首次报道,研究结果为进一步探究GPR161基因在鸟类中的生理效应提供参考。
英文摘要:G protein-coupled receptor 161 (GPR161) is a member of GPCRs, and this orphan receptor plays an important role in the lens development and neurulation of mammals. Recent study showed that the structure of GPR161 was similar with scaffolding proteins and thus may had a potential function in signal transduction. Using chicken as experimental model, the cDNA sequence, molecular evolution and tissue expression of chicken GPR161 gene were investigated in this study. The full-length coding sequence of chicken GPR161 gene was 1 566 bp in length and encoding 521 amino acids. Sequence analysis revealed that GPR161 of chicken had high sequence similarity with that of human (83.0%), mouse (82.6%) or zebrafish (65.8%). Molecular evolutionary genetical analysis showed that the relationship of GPR161 among chicken, human and mouse were closer than that of chicken and zebrafish. The result of real-time quantitative PCR showed that the mRNA level of GPR161 gene was abundant in testis or ovary, brain, heart and muscle. Our data provide a basis for the study of the physiological function of GPR161 gene in birds.
2017,36(6): 632-638 收稿日期:2017-05-02
DOI:10.11984/j.issn.1000-7083.20170140
分类号:Q959.7;Q78
作者简介:祝国强(1992-),硕士,主要从事动物分子遗传研究,E-mail:872139941@qq.com
*通讯作者:李娟,E-mail:lijuanscuhk@163.com
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