刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610064
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2017年第36卷第4期

猫科动物DNA条形码及线粒体假基因对物种鉴定的影响
DNA Barcoding in Felidae, and the Effects of Nuclear Mitochondrial Pseudogenes on Species Identification
蔡延森1, 李佳凌2, 赵矫1, 张亮3*
点击:142次 下载:0次
DOI:
作者单位:1. 西南医科大学医学细胞生物学与遗传学教研室, 四川泸州 646000;
2. 西南医科大学形态学研究室, 四川泸州 646000;
3. 成都大熊猫繁育研究基地, 四川省濒危野生动物保护生物学重点实验室, 成都 610081
中文关键字:COⅠ;猫科;线粒体假基因
英文关键字:COⅠ; Felidae; nuclear mitochondrial pseudogenes
中文摘要:在多种动物类群中,基于线粒体细胞色素c氧化酶亚基Ⅰ(COⅠ)基因的DNA条形码是一种高效的物种鉴别手段,然而猫科Felidae动物中广泛存在的线粒体假基因可能影响DNA条形码的有效性。本研究共涉及猫科动物12属25种119个样本。采用3对条形码通用引物对6属11种29个猫科动物样本进行了扩增及测序。结果3个样本扩增失败,8个样本得到假基因,18个样本获得了条形码序列。结合另外93条猫科动物条形码序列(源自BOLD Systems),采用Kimura 2-parameter模型计算遗传距离,构建Neighbor-Joining(NJ)树。结果显示,遗传距离种内为0%~8.1%,平均0.8%;种间为1.4%~13.1%,平均8.7%;属间为8.2%~21.8%,平均15.1%。NJ树显示,除3个种外,其余物种均以极高的置信度(99%)形成单系分支。而假基因序列有些可以单独形成分支,有些夹杂在COⅠ序列形成的分支中,对物种鉴定产生干扰。
英文摘要:In a variety of animal species, DNA barcoding based on mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene is an efficient tool for species identification. However, the presence of large mitochondrial pseudogenes in felids may affect the effectiveness of DNA barcoding. In this study, 119 felid samples from 25 species of 12 genera were analyzed.3 pairs of universal primers were used to amplify and sequence the COⅠ genes of 29 felid samples that belonged to 11 species from 6 genera. The results showed that the amplification was failed in 3 samples, nuclear mitochondrial psedogenes (numts) were obtained in 8 samples, and barcode sequences were derived in 18 samples. Combined with the other 93 felid barcode sequences (derived from BOLD Systems), Kimura 2-parameter model was used to calculate the genetic distance and to construct Neighbor-Joining (NJ) tree. The genetic distance within species were 0%-8.1%, with an average of 0.8%; the interspecific genetic distance were 1.4%-13.1%, with an average of 8.7%; and the genetic distance within family were 8.2%-21.8%, with an average of about 15.1%. The NJ tree showed that most of the species formed a monophyletic clade with high confidence (99%), except 3 species; some of the numts sequences could form a clade on NJ tree, and the others were inserted in the branches of the COⅠ sequences, which may compromise the species identification.
2017,36(4): 425-430 收稿日期:2017-03-13
DOI:10.11984/j.issn.1000-7083.20170079
分类号:Q959.8
基金项目:四川省教育厅科研项目(12ZA242)
作者简介:蔡延森(1982-),男,硕士,讲师,E-mail:cys_cys@aliyun.com
*通讯作者:张亮,E-mail:zliang@panda.org.cn
参考文献:
蔡延森, 刘芹, 王译伟, 等. 2014. 四川宜宾产桃花水母的线粒体COⅠ序列片段分析[J]. 泸州医学院学报, (1):79-82.
蔡延森, 张修月, 岳碧松, 等. 2009. 我国8种猛禽的DNA条形码技术研究[J]. 四川动物, 28(3):334-340.
周志军, 尚娜, 常岩林, 等. 2014. DNA条形码揭示日本纺织娘(Mecopoda niponensis)个体内和个体间的序列变异[J]. 生物技术通报, (5):129-136.
Brown WM, Prager EM, Wang A, et al. 1982. Mitochondrial DNA sequences of primates:tempo and mode of evolution[J]. Journal of Molecular Evolution, 18(4):225-239.
Cai YS, Zhang L, Wang Y, et al. 2016. Identification of deer species (Cervidae, Cetartiodactyla) in China using mitochondrial cytochrome c oxidase subunit Ⅰ (mtDNA COⅠ)[J]. Mitochondrial DNA Part A:the Journal of DNA Mappong Sequencing, and Analysis, 27(6):4240-4243.
Cai YS, Zhang L, Shen FJ, et al. 2011. DNA barcoding of 18 species of Bovida[J]. Chinese Science Bulletin, 56(2):164-168.
Hebert PDN, Cywinska A, Ball SL, et al. 2003. Biological identifications through DNA barcodes[J]. Proceedings of the Royal Society B-Biological Sciences, 270:313-321.
Hebert PDN, Stoeckle MY, Zemlak TS, et al. 2004. Identification of birds through DNA barcodes[J]. PLoS Biology, 2(10):e312.
Kress WJ, Garcı'a-Robledo C, Uriarte M, et al. 2015. DNA barcodes for ecology, evolution, and conservation[J]. Trends in Ecology & Evolution, 30(1):25-35.
Li J, Cui Y, Jiang J, et al. 2017. Applying DNA barcoding to conservation practice:a case study of endangered birds and large mammals in China[J]. Biodiversity & Conservation, 26(3):653-668.
Li WH, Gojobori T, Nei M. 1981. Pseudogenes as a paradigm of neutral evolution[J]. Nature, 292(5820):237-239.
Lopez JV, Yuhki N, Masuda R, et al. 1994. Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat[J]. Journal of Molecular Evolution, 39(2):174-190.
Mukherjee S, Appel A, Duckworth JW, et al. 2016. Prionailurus viverrinus[DB/OL]. The IUCN Red List of Threatened Species 2016:e.T18150A50662615.
Wozencraft WC. 2005. Mammal species of the world:a taxonomic and geographic reference (3rd Ed.)[M]. Baltimore:Johns Hopkins University Press:536-537.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2018四川动物》编辑部 蜀ICP备08107403号-3
您是本站第5963388名访问者

川公网安备 51010702000173号