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牦牛和雄性不育犏牛睾丸PPP2CA、PME-1基因mRNA水平的比较
Comparison of mRNA Levels of PPP2CA and PME-1 Genes in the Testes of Yaks and Sterile Cattle-yaks
任亮1,2, 付伟1, 金素钰1, 黄林1, 李彩霞1, 郑玉才1*
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DOI:10.11984/j.issn.1000-7083.20160337
作者单位:1. 西南民族大学生命科学与技术学院, 成都 610041;
2. 四川省广元市剑阁县动物疫病预防控制中心, 四川广元 628300
中文关键字:牦牛;犏牛;杂交雄性不育;蛋白磷酸酶2A;蛋白磷酸甲酯酶-1
英文关键字:yak; cattle-yak; hybrid male sterility; protein phosphatase 2A; protein methylesterase 1
中文摘要:蛋白磷酸酶2A(PP2A)参与细胞中蛋白质的可逆磷酸化作用,在磷酸化信号通路调控方面具有重要功能,其活性受蛋白磷酸甲酯酶-1(PME-1)和其他因素的调控。为阐明牦牛杂交雄性不育的分子机制,本研究从牦牛睾丸中克隆了PP2A催化亚基α基因(PPP2CA)和PME-1基因cDNA序列,编码区长度分别为930 bp和1 143 bp,编码的氨基酸序列保守;定量PCR检测显示,犏牛(n=7)睾丸中PPP2CAPME-1基因的mRNA水平均极显著低于牦牛(n=13,P<0.01)。根据本研究结果,推测犏牛睾丸中这2个基因的显著下调可能会影响一些重要信号通路,且与犏牛雄性不育有关。
英文摘要:Protein phosphatase 2A (PP2A) mainly functions in protein reversible phosphorylation, and plays a central role in phosphorylation-dependent signaling pathways. Its activity is regulated by protein methylesterase 1 (PME-1) and other factors. To elucidate the molecular mechanism of hybrid male sterility of yaks, we cloned cDNA sequences of catalytic subunit α (PPP2CA) of PP2A and PME-1 genes. The coding regions of yak PPP2CA and PME-1 genes were 930 bp and 1 143 bp, respectively. The deduced amino acid sequences were conserved. Quantitative PCR assay showed that cattle-yaks (n=7) contained dramatically reduced mRNA levels of PPP2CA and PME-1 genes in testes than yaks (n=13, P<0.01). The present results suggested that the reduced mRNA levels of the two genes in the testes might influence some important signaling pathways and associate with hybrid male sterility of cattle-yaks.
2017,36(3): 306-310 收稿日期:2016-11-30
DOI:10.11984/j.issn.1000-7083.20160337
分类号:Q311
基金项目:国家自然科学基金项目(31240053)
作者简介:任亮(1988-),助理兽医师,硕士,研究方向:生化分析及分子生物学方向
*通讯作者:郑玉才,教授,博士,E-mail:yucaizheng65@hotmail.com
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