刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610064
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2017年第36卷第2期

美洲大蠊醇提物对大鼠抗氧化应激的影响
Influence of Periplaneta americana Extract on Antioxidant Stress in Rats
隋世燕, 葛亚男, 徐取尉, 苏晶晶
点击:305次 下载:0次
DOI:10.11984/j.issn.1000-7083.20160279
作者单位:大理大学农学与生物科学学院, 云南大理 671003
中文关键字:美洲大蠊醇提物;氧化应激;基因表达;血清;卵巢;大鼠
英文关键字:Periplaneta americana alcohol extract; oxidative stress; gene expression; serum; ovary; rats
中文摘要:目的 研究美洲大蠊Periplaneta americana醇提物对大鼠血清和卵巢抗氧化指标的影响。方法 17只成年雌性SD大鼠适应饲养7 d后,随机分为3组:对照组(9 mL·kg-1,生理盐水)5只、低剂量组(9 mL·kg-1,0.03 g·mL-1美洲大蠊醇提物)6只、高剂量组(9 mL·kg-1,0.09 g·mL-1美洲大蠊醇提物)6只。每2天灌胃1次,共饲养30 d。实验结束后,采集大鼠血清和卵巢,检测血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、一氧化氮(NO)、谷胱甘肽过氧化物酶(GSH-PX)指标和卵巢中SOD、GSH-PX、CAT基因表达的变化。结果 与对照组相比,高剂量组(P=0.085)、低剂量组(P=0.091)血清中的NO含量均有降低的趋势;高剂量和低剂量的美洲大蠊醇提物均显著提高了大鼠血清中的SOD含量(P<0.05),同时,高剂量组血清中的MDA含量显著降低(P<0.05);此外,高剂量组和低剂量组卵巢SOD基因的表达显著升高(P<0.05)。结论 美洲大蠊醇提物升高了大鼠血清SOD的含量,降低了NO和MDA的含量,同时,还提高了卵巢SOD基因的表达,说明美洲大蠊醇提物对大鼠卵巢具有一定的抗氧化应激功能。
英文摘要:Objective To study the effect of Periplaneta americana alcohol extract on antioxidant index of serum and ovary in rats. Methods A total of 17 adult female SD rats were randomly assigned to 3 groups after a week of adaption in the laboratorial environment. The rats of control group (n=5) were fed with physiological saline (9 mL·kg-1) through stomach perfusion. The rats of experimental groups (6 per group) were treated with low-dose (9 mL·kg-1, 0.03 g·mL-1) and high-dose (9 mL·kg-1, 0.09 g·mL-1) P. americana alcohol extract as described above. Rats were treated every other day for 30 days. Subsequently, the serum and ovaries of rats were collected, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), nitric oxide (NO), glutathione (GSH-PX) in the serum, the expression of gene SOD, GSH-PX and CAT in the ovary were detected. Results Compared with control group, the NO content in rats serums of high-dose group (P=0.085) and low-dose group (P=0.091) showed a decreasing trend but not significant; the content of SOD in rats serums were significantly increased (P<0.05). Moreover, the content of MDA in rats serums were significantly decreased in the high-dose group (P<0.05). In addition, P. americana alcohol extract treatment significantly increased the expression of SOD gene in ovary (P<0.05). Conclusion P. americana alcohol extract can significantly increase the content of antioxidant index SOD and reduce levels of the oxidative stress indicators NO and MDA, and can also significantly increase the expression of SOD gene in ovary. Therefore, P. americana alcohol extract has a certain antioxidant stress function to the ovary of rats.
2017,36(2): 198-202 收稿日期:2016-10-14
DOI:10.11984/j.issn.1000-7083.20160279
分类号:Q95-33
基金项目:云南省教育厅项目(2014C110Y);大理大学博士科研启动基金项目(KYBS201406)
作者简介:隋世燕(1981-),男,博士,讲师,主要从事动物营养与繁殖方面的研究,E-mail:sysui569@163.com
参考文献:
戴云, 曾茗, 项朋志. 2005. 蜚蠊的药用价值[J]. 中药材, 28(9):848-850.
甘平, 张旭强, 何旭, 等. 2011. 美洲大蠊醇提物对小鼠急性肝损伤的保护作用[J]. 现代药物与临床, 26(2):123-128.
何正春, 胡明辉, 王晓雨, 等. 2009. 美洲大蠊提取物对3株人及小鼠白血病细胞的细胞毒性研究[J]. 云南中医中药杂志, 30(5):56-57.
焦春香, 张成桂, 刘光明, 等. 2011. 美洲大蠊提取物中抗衰老活性部位抗氧化活性的初步分析[J]. 时珍国医国药, 22(6):1389-1391.
吴军, 赵凤鸣, 王明艳, 等. 2007. 四君子汤对环磷酰胺造模小鼠血清中SOD、GSH、CAT和MDA水平的影响[J]. 辽宁中医杂志, 34(8):1168-1169.
邹俊波, 桑文涛, 王芳, 等. 2016. 美洲大蠊提取物对乙醇致小鼠急性胃溃疡的预防作用[J]. 中成药, 38(11):2325-2331.
Agarwal A, Gupta S, Sharma RK. 2005. Role of oxidative stress in female reproduction[J]. Reproductive Biology and Endocrinology, 3(28):1-21.
Agarwal A, Gupta S, Sikka S. 2006. The role of free radicals and antioxidants in reproduction[J]. Current Opinion in Obstetrics and Gynecology, 18(3):325-332.
Agarwal A, Saleh RA, Bedaiwy MA. 2003. Role of reactive oxygen species in the pathophysiology of human reproduction[J]. Fertility and Sterility, 79(4):829-843.
Aitken RJ,Koppers AJ. 2011. Apoptosis and DNA damage in human spermatozoa[J]. Asian Journal of Andrology, 13(1):36-42.
Bagchi D, Garg A, Krohn RL, et al. 1997. Oxygen free radical scavenging abilities of vitamins C and E and a grape seed proanthocyanidin extract in vitro[J]. Research Communications in Molecular Pathology and Pharmacology, 95(2):179-189.
Bausenwein J, Serke H, Eberle K, et al. 2010. Elevated levels of oxidized low-density lipoprotein and of catalase activity in follicular fluid of obese women[J]. Molecular Human Reproduction, 16(2):117-124.
Goud AP, Goud PT, Diamond MP, et al. 2008. Reactive oxygen species and oocyte aging:role of superoxide, hydrogen peroxide, and hypochlorous acid[J]. Free Radical Biology and Medicine, 44(7):1295-1304.
Husain K, Whitworth C, Somani SM, et al. 2001. Carboplatin-induced oxidative stress in rat cochlea[J]. Hearing Research, 159(1-2):14-22.
Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 25(4):402-408.
Oyawoye O, Abdel Gadir A, Garner A, et al. 2003. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF:relationship to outcome[J]. Human Reproduction, 18(11):2270-2274.
Riva C, Binelli A, Cogni D, et al. 2007. Evaluation of DNA damage induced by decabromodiphenyl ether (BDE-209) in hemocytes of dreissena polymorpha using the comet and micronucleus assays[J]. Environmental and Molecular Mutagenesis, 48(9):735-743.
Rizzo A, Roscino MT, Binetti F, et al. 2012. Roles of reactive oxygen species in female reproduction[J]. Reproduction in Domestic Animals, 47(2):344-352.
Sikka SC. 2001. Relative impact of oxidative stress on male reproductive function[J]. Current Medicinal Chemistry, 8(7):851-862.
Wathes DC, Abayasekara DR, Aitken RJ. 2007. Polyunsaturated fatty acids in male and female reproduction[J]. Biology of Reproduction, 77(2):190-201.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2018四川动物》编辑部 蜀ICP备08107403号-3
您是本站第6376005名访问者

川公网安备 51010702000173号