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冷驯化对中缅树鼩PPARα、COXⅡ及PGC-1α基因表达量的影响
Effect of Cold Exposure on the Expressions of PPARα, COXⅡ and PGC-1α in Tupaia belangeri
侯东敏1, 章迪2, 余婷婷1, 王政昆1, 高文荣1, 朱万龙1*
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DOI:10.11984/j.issn.1000-7083.20160232
作者单位:1. 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室, 云南师范大学生命科学学院, 昆明 650500;
2. 云南经济管理学院, 昆明 650106
中文关键字:中缅树鼩;脂肪组织;冷驯化
英文关键字:Tupaia belangeri; adipose tissue; cold exposure
中文摘要:为了研究冷驯化条件下中缅树鼩Tupaia belangeri的脂肪组织是否会转化,本研究测定了冷驯化条件下中缅树鼩脂肪组织质量,脂肪转化因子过氧化物酶体增殖激活受体α(PPARα)、环氧化酶-2(COXⅡ)及过氧化物酶体增殖物受体γ共激活因子1α(PGC-1α)基因表达量的变化。结果表明:中缅树鼩冷驯化组无论是褐色脂肪组织(BAT)质量,还是大网膜白色脂肪组织(WAT)质量均较对照组显著增加(P<0.01),脂肪转化因子PPARα、COXⅡ及PGC-1α基因表达量也显著上调(P<0.01)。以上结果说明中缅树鼩WAT中PPARα、COXⅡ、PGC-1α基因表达量的增加可能诱导了WAT细胞褐变,进而向BAT细胞转化和提高BAT中解偶联蛋白1的表达。
英文摘要:In order to investigate whether the adipose tissue of Tupaia belangeri would be transformed under cold acclimation, the mass of adipose tissue, changes of peroxisome proliferator-activated receptor α (PPARα), cyclooxygenase-2 (COXⅡ) and peroxisome proliferators-activated receptor-γ coactivator-1 (PGC-1α) genes were measured in the present study. The results showed that mass of brown adipose tissue and omental white adipose tissue, the mRNA expressions of PPARα, COXⅡ and PGC-1α genes were increased extreme significantly under cold exposure compared with control (P<0.01). And these indicated that the increased expressions of PPARα, COXⅡ and PGC-1α genes under cold condition may cause the transformation of white adipose tissue to browning beige adipocyte, which then transformed to brown adipose tissue and increase the level of uncoupling protein 1 in brown adipose tissue.
2017,36(1): 34-38 收稿日期:2016-08-31
DOI:10.11984/j.issn.1000-7083.20160232
分类号:Q95-33
基金项目:国家自然科学基金项目(31660121);联合支持国家计划项目(2015GA008);云南省科技计划重点项目(2016FA045)
作者简介:侯东敏(1992-),男,硕士研究生,研究方向:动物生理生态,E-mail:houl92@163.com
*通讯作者:朱万龙,男,副教授,主要从事动物生理生态研究,E-mail:zwl_8307@163.com
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