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基于RNA-seq的黄蜻头部转录组测序与分析
Transcriptome Analysis of Pantala flavescens (Fabricius) Based on RNA-seq
葛怡情, 曹玲珍*
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作者单位:江西师范大学生命科学学院, 鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330000
中文关键字:黄蜻;转录组;测序;基因分析;功能注释
英文关键字:Pantala flavescens (Fabricius); transcriptome; sequencing; gene analysis; function annotation
中文摘要:利用RNA-seq技术对黄蜻Pantala flavescens (Fabricius)头部细胞的全部转录本进行测序、功能分析。提取黄蜻头部总RNA,反转录得到cDNA,在Illumina平台上进行测序,经拼接、组装、聚类后获得全部unigenes,并将所得的unigenes与数据库比对,对其进行功能注释和分类。总共得到47 039 040条碱基序列,包含了7 102 895 040个碱基序列信息,碱基G和C的数量总和占总碱基数量的44.05%。将测序结果进行组装,组装所得到的unigenes为34 406 502条,对所得到的unigenes进行不同的数据库注释,共有44 499条unigenes被注释到数据库,其中有大量unigenes与代谢过程、结合活性、催化活性和细胞进程有关。与COG数据库比对,根据功能可以分为26类,其中信号转导机制最为丰富。与KEGG数据库比对,线粒体基因在所测样本中最为丰富,与KEGG ORTHOLOGY注释后,代谢活动基因最为丰富。这些与线粒体和代谢有关的基因与我们研究中与黄蜻迁飞基因相关,为后续研究奠定了基础。
英文摘要:The total transcripts of Pantala flavescens (Fabricius) were obtained by RNA-seq followed by functional analysis. The head total RNA of P. flavescens (Fabricius) was extracted and then sequenced by Illumina platform. A total of 47 039 040 reads containing 7 102 895 040 bp were generated. There were 34 406 502 unigenes with GC content of 44.05% after de novo assembly. All the unigenes were annotated based on different databases, and 44 499 unigenes were annotated. In this study, all the assembled unigenes could be broadly divided into biological processes, cellular components and molecular function categories of 60 branches by gene ontology, including metabolic process, binding, catalytic activity and cellular process. Unigenes were further annotated based on COG category, which could be grouped into 26 functional categories. The results of KEGG prediction suggested that mitochondrial genes were the most abundant in the tested samples. And after KEGG ORTHOLOGY annotation, the most abundant genes were metabolic-related. The genes of mitochondria and metabolism were related with the migration mechanism of P. flavescens (Fabricius).
2016,35(6): 852-859 收稿日期:2016-08-23
DOI:10.11984/j.issn.1000-7083.20160227
分类号:Q963
基金项目:鄱阳湖湿地与流域研究教育部重点实验室(江西师范大学)开放基金项目(PK2013001);江西省教育厅课题“基于线粒体分子标记的江西黄蜻遗传多样性研究”项目(7357)
作者简介:葛怡情(1994-),本科生,从事动物学研究,E-mail:1757883815@qq.com
*通讯作者:曹玲珍,E-mail:clzclz1011@163.com
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