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川陕哲罗鲑精子超微结构及形态学研究
Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa
郭威1,2,3, 厉萍3, 杨焕超3,4, 杜浩3, 吴金明3, 危起伟1,3,4*
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DOI:
作者单位:1. 中国科学院水生生物研究所, 武汉 430072;
2. 中国科学院大学, 北京 100039;
3. 中国水产科学研究院长江水产研究所农业部淡水生物多样性保护重点实验室, 武汉 430223;
4. 华中农业大学水产学院, 武汉 430070
中文关键字:川陕哲罗鲑;精子;超微结构
英文关键字:Hucho bleekeri Kimura; spermatozoa; ultrastructure
中文摘要:对川陕哲罗鲑Hucho bleekeri Kimura精子采用扫描电子显微镜及透射电子显微镜进行观察,结果显示:精子由头部、中片和尾部组成;精子全长41.07 μm±2.18 μm,头部长2.76 μm±0.15 μm,头部前端和后端的宽度分别为1.88 μm±0.18 μm和2.08 μm±0.20 μm;尾部长34.74 μm±5.01 μm。头部呈卵圆形,无顶体,主要由细胞核组成;中片由1个不规则的圆球状线粒体及袖套结构组成;线粒体直径为0.82 μm±0.08 μm;尾部呈细长形,并由一个过渡区域分为前端和末端,尾部内部主要由轴丝组成,轴丝为典型的“9+2”结构,外部有不对称性分布的侧鳍结构。结果表明,川陕哲罗鲑精子类型较为原始,属于硬骨鱼类中的TypeⅠ类型。
英文摘要:This study was conducted to investigate the morphology and ultrastructure of Hucho bleekeri Kimura spermatozoa through scanning and transmission electron microscopy. The results revealed that the spermatozoa can be differentiated into three major parts: a head, a mid-piece, and a flagellum. The mean length of the spermatozoa was 41.07 μm±2.18 μm, with a spherical head length of 2.76 μm±0.15 μm and a flagellum length of 34.74 μm±5.01 μm. The mean anterior and posterior head widths were 1.88 μm±0.18 μm and 2.08 μm±0.20 μm, respectively. The head was spherical without acrosome, which was composed of nucleus. The short mid-piece was located laterally to the nucleus and possessed just one spherical mitochondrion with a mean diameter of 0.82 μm±0.08 μm. The spermatozoa flagellum was long and cylindrical, and could be separated into two parts. The axoneme composed the typical "9+2" microtubular doublet structure and was enclosed by the cell membrane. The flagellum had short irregular side-fins. This study confirmed that H. bleekeri Kimura spermatozoa can be categorized as teleostean Type Ⅰ; "primitive" or "ect-aquasperm type" spermatozoa.
2016,35(4): 550-555 收稿日期:2016-03-07
DOI:10.11984/j.issn.1000-7083.20160047
分类号:Q959.4
基金项目:公益性行业(农业)科研专项(201203086);国家自然科学基金青年项目(31402301);中国水产科学研究院基本科研业务费(2014A07XK05)
作者简介:郭威(1989-),男,硕士研究生,研究方向为濒危鱼类保护,E-mail:guowei612@escience.cn
*通讯作者:危起伟,E-mail:weiqw@yfi.ac.cn
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