To explore the feasibility of establishing Alzheimer's disease (AD) animal model with tree shrew (Tupaia belangeri
30 adult male tree shrews were randomly divided into three groups. Intraperitoneal injection of D-galactose (D-gal) was made to the model group causing acute aging, then the mixture of amyloid protein β1~42 fragment (Aβ1~42) and ibotenic acid (IBO) was injected once into the bilateral hippocampus of the tree shrews; all those drugs were replaced with normal saline in equivalent doses for the control group, while no treatment was done to the blank group. Then behavioral test was conducted to the three groups of tree shrew through Morris water maze, and pathological detection and analysis was made through HE staining and silver impregnation staining. Result
Morris water maze test suggested that significant learning and memory dysfunction occurred on the model tree shrews. The pathological detection indicated that significant gliosis and neurofibrillary tangles occurred at the cerebral hippocampus of the model group. No significant pathological changes happened on the control group and the blank group. Conclusion
Intraperitoneal injection of D-gal and injection of the mixture of Aβ1~42 and IBO into the bilateral hippocampus would cause decline in learning and memory ability of the tree shrews, as well as gliosis and neurofibrillary tangles, thus it was a feasible modeling method of AD model with tree shrew.
2016,(35): 270-274 收稿日期：2015-09-17
作者简介：欧阳轶强(1977-), 博士研究生, 从事人类疾病动物模型研究
*通讯作者：郭松超, 博士生导师, 研究方向:营养与疾病, 实验动物学, E-mail:email@example.com
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