The expression of mEppin
gene in different developing stages of male mice testes was examined by real time fluorescent quantitative PCR (qPCR) assay. This study aims to provide some informations regarding the association of mEppin
gene expression with male reproduction. Methods
The total RNA was extracted from testes of 18 ICR mice at different developing stages (embryo, 50 d-old, and 90 d-old). The expression levels of mEppin
mRNA in testis were detected by qPCR. Results
The expression levels of mEppin
gene among three developing stages of testes were significantly different. High expression levels of mEppin
gene were detected in the testes of 50 d-old and 90 d-old mice, whereas no expression was detected in embryonic testes. The expression level of mEppin
gene in the testis of 90 d-old mice was 2.65-fold higher than that in 50 d-old. Conclusion
gene expression in developing testes is stage-dependent in mice.
2016,(35): 256-260 收稿日期：2015-09-02
作者简介：唐东红(1966-), 女, 主任技师, 研究方向:疾病动物模型, E-mail:firstname.lastname@example.org, email@example.com
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