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不同发育阶段雄性小鼠睾丸组织mEppin基因mRNA的表达差异研究
Expression of mEppin Gene in Different Developing Stages of Male Mice Testes
唐东红1, 叶尤松1, 李哲丽1, 李司2, 杨光蕊2, 李桂珍2, 李润萍2, 彭波2
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DOI:
作者单位:1. 中国医学科学院/北京协和医学院医学生物学研究所, 昆明 650118;
2. 云南中医学院, 昆明 650200
中文关键字:小鼠;mEppin基因;mRNA表达差异;实时荧光定量PCR
英文关键字:mouse; mEppin gene; different mRNA expression; real-time fluorescent quantitative PCR
中文摘要:目的 实时荧光定量PCR检测雄性小鼠不同发育阶段睾丸组织中mEppin基因的mRNA表达差异,为探究mEppin基因表达与雄性生殖相关性提供生物学数据。方法 分别选取胚胎期、50日龄、90日龄雄性ICR小鼠各6只,共18只,取各组小鼠睾丸组织提取总RNA,实时荧光定量PCR方法检测mEppin基因mRNA表达水平。结果 不同发育阶段小鼠睾丸mEppin基因表达水平差异明显。胚胎组小鼠中没有检测到mEppin基因mRNA表达,50日龄小鼠和90日龄小鼠中均检测到mEppin基因mRNA有较高表达水平。其中,90日龄小鼠睾丸中mEppin基因mRNA的相对表达水平是50 日龄小鼠的2.65倍。结论 本研究结果表明mEppin基因mRNA的表达在小鼠睾丸组织中具阶段特异性。
英文摘要:Objective The expression of mEppin gene in different developing stages of male mice testes was examined by real time fluorescent quantitative PCR (qPCR) assay. This study aims to provide some informations regarding the association of mEppin gene expression with male reproduction. Methods The total RNA was extracted from testes of 18 ICR mice at different developing stages (embryo, 50 d-old, and 90 d-old). The expression levels of mEppin mRNA in testis were detected by qPCR. Results The expression levels of mEppin gene among three developing stages of testes were significantly different. High expression levels of mEppin gene were detected in the testes of 50 d-old and 90 d-old mice, whereas no expression was detected in embryonic testes. The expression level of mEppin gene in the testis of 90 d-old mice was 2.65-fold higher than that in 50 d-old. Conclusion The mEppin gene expression in developing testes is stage-dependent in mice.
2016,(35): 256-260 收稿日期:2015-09-02
DOI:10.11984/j.issn.1000-7083.20150268
分类号:Q78
作者简介:唐东红(1966-), 女, 主任技师, 研究方向:疾病动物模型, E-mail:tdh@imbcams.com.cn, 783849021@qq.com
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