刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610065
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2015年第34卷第6期

家鸡G蛋白偶联受体139基因的结构、序列及表达特征分析
Characterization of the Structure and Tissue Expression of Chicken G Protein-coupled Receptor (GPR139) Gene
黄思邈, 黄龙, 莫春横, 李娟, 王亚军*
点击:1034次 下载:10次
DOI:
作者单位:四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 成都610064
中文关键字:家鸡;GPR139;G蛋白偶联受体;中枢神经系统
英文关键字:chicken; GPR139; G protein-coupled receptor; central nervous system
中文摘要:G蛋白偶联受体139(GPR139)是一种孤儿受体。在脊椎动物中,针对其结构、表达和功能的研究相对缺乏。本文以家鸡大脑cDNA为模板,采用PCR方法,扩增并克隆得到家鸡GPR139(cGPR139)基因。结果显示:家鸡GPR139基因cDNA由2个外显子组成;ORF区域长1056 bp,编码351个氨基酸的前体蛋白,在其第三跨膜区末端包含D-R-Y基序,属于G蛋白偶联受体家族视紫红质亚家族。将家鸡GPR139前体蛋白与人、大鼠、小鼠GPR139前体蛋白的氨基酸序列进行比对,分别具有91.78%、89.17%、89.17%的相似度。采用RT-PCR方法,本研究也检测了家鸡GPR139的组织表达情况。结果显示:家鸡GPR139基因在大脑、中脑、小脑、后脑、下丘脑及垂体中表达量较高,而在卵巢、精巢、肺、肾脏、肌肉组织中,GPR139基因仅有微弱表达。这些结果首次揭示了GPR139基因在非哺乳动物(家鸡)中的结构特征和组织表达特点,为探究其在家鸡中的生理功能奠定一些基础。
英文摘要:G protein-coupled receptor 139 (GPR139) is an orphan receptor identified in vertebrates, however, the information regarding its structure, expression and functions remains limited. In this study, the full-length cDNA of chicken GPR139 (cGPR139) genes was cloned by PCR. Chicken GPR139 cDNA consisted of two exons, and its open reading frame (1056 bp) encoded a protein precursor of 351 amino acids, which shared high amino acid sequence identity with that of human (91.78%), rat (89.17%) and mouse (89.17%). The third transmembrane domain of cGPR139 contained a D-R-Y motif, which was typical for rhodopsin subfamily of G protein-coupled receptors. In addition, the expression of GPR139 gene was examined in adult chicken tissues by RT-PCR. The results showed that chicken GPR139 was highly expressed in the pituitary and various brain regions, including telencephalon, midbrain, cerebellum, hindbrain and hypothalamus, but weakly expressed in the ovary, testis, lung, kidney and muscle. The results from present study will pave the way for further investigation of the roles of GPR139 in chickens.
2015,34(6): 869-874 收稿日期:2015-04-12
DOI:10.11984/j.issn.1000-7083.20150177
分类号:Q78
基金项目:国家自然科学基金项目(31271325,31272436)
作者简介:黄思邈,细胞生物学硕士研究生,E-mail:hsm900@163.com
*通讯作者:王亚军,E-mail:cdwyjhk@gmail.com
参考文献:
Andrew LH, Colin RG. 2002. The druggable genome[J]. Drug Discovery, 1(9): 727-730.
Ayako M, Shun-ichiro M, Mamoru N, et al. 2005. Molecular cloning and characterization of a novel Gq-coupled orphan receptor GPRg1 exclusively expressed in the central nervous system[J]. Biochemical and Biophysical Research Communications, 331(1): 363-369.
Chung S, Funakoshi T, Civelli O. 2008. Orphan GPCR research[J]. British Journal of Pharmacology, 153 suppl 1(S1): S339-S346.
David EIG, Helgi BS, Robert F. 2005. Nine new human Rhodopsin family G-protein coupled receptors: identification, sequence characterisation and evolutionary relationship[J]. Biochimica et Biophysica Acta, 1722(3): 235-246.
Davide L, Maria PA. 2008. Deorphanisation of G protein-coupled receptors: a tool to provide new insights in nervous system pathophysiology and new targets for psycho-active drugs[J]. Neurochemistry International, 52(3): 339-351.
Feng S, Jing KS, Danqi C, et al. 2011. Discovery and SAR of a series of agonists at orphan G protein-coupled receptor 139[J]. ACS Medicinal Chemistry Letters, 2(4): 303-306.
Joel B, Jean PP. 1999. Molecular tinkering of G protein-coupled receptors: an evolutionary success[J]. The EMBO Journal, 18: 1723-1729.
Jurgen D. 2000. Drug fiscovery: a historical perspective[J]. Science, 287(5460): 1960-1964.
Kavita KK, Kaiser J. 2014. Sequence-structure based phylogeny of GPCR class A rhodopsin receptors[J]. Molecular Phylogenetics and Evolution, 74: 66-96.
Kristen LP, Richard TP, Robert JL. 2002. Seven-transmembrane receptors[J]. Molecular Cell Biology, 3(9): 639-650.
Liaoyuan AH, Pauline MT, Nima KE, et al. 2009. Identification of surrogate agonists and antagonists for orphan G-protein-coupled receptor GPR139[J]. Journal of Biomolecular Screening, 14(7): 789-797.
Melvin IS, Michael PS, Narasimhan G. 1991. Diversity of G proteins in signal transduction[J]. Science, 252(5007): 802-808.
Olivier C, Yumiko S, Zhiwei W, et al. 2006. Orphan GPCRs and their ligands[J]. Pharmacology & Therapeutics, 110: 525-532.
Raise A, Stefanie W, Ralf J. 2015. Hunting for the function of orphan GPCRs-beyond the search for the endogenous ligand[J]. British Journal of Pharmacology, 172(13): 3218-3228.
Robert F, Malin CL, Lars-gustav L, et al. 2003a. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints[J]. Molecular Pharmacology, 63(6): 1256-1272.
Robert F, Par JH, David EIG, et al. 2003b. Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives[J]. Febs Letters, 554(3): 381-388.
Thóra KB, David EG, Sofia HH, et al. 2006. Comprehensive repertoire and phylogenetic analysis of the G protein-coupled receptors in human and mouse[J]. Genomics, 88(3): 263-273.
Ulrik G. 2000. Uncovering molecular mechanisms involved in activation of G protein-coupled receptors[J]. Endorine Reviews, 21(1): 137-166.
Ute S, Irm HB, Jens U, et al. 2006. Characterisation and differential expression of two very closely related G-protein-coupled receptors, GPR139 and GPR142, in mouse tissue and during mouse development[J]. Neuropharmacology, 50(4): 512-520.
Vignir I, Kirsten BA, Christoph B, et al. 2014. Computer-aided discovery of aromatic L-α-amino acids as agonists of the orphan G protein-coupled receptor GPR139[J]. Journal of Chemical Information & Modeling, 54(6): 1553-1557.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2019四川动物》编辑部 蜀ICP备08107403号-3
您是本站第7567261名访问者

川公网安备 51010702000173号