Isolation and Antimicrobial Resistance of Bacteria from Intestinal Tract of Giant Panda
作者单位：(1. 四川农业大学都江堰校区微生物学实验室，森林资源保护与利用实验室，四川都江堰611830；2. 四川农业大学资源环境学院，成都611130；3. 中国保护大熊猫研究中心雅安碧峰峡基地，四川雅安625000)
英文关键字：giant panda; fungi; Escherichia coli; Salmonella; isolation and identification; antibiotic resistance
英文摘要：Feces samples of 64 giant pandas were collected from China's Panda Research Center Bifengxia base. Escherichia coli and Salmonella sp. were isolated using selective medium. Isolates were identified by morphological characteristics and confirmed by 16S rDNA sequence analysis. Fungi were isolated using PDA medium, and identified with universal primer (ITS4 and ITS5). The susceptibility of isolates to eighteen antibiotics was performed according to the standard disk diffusion method recommended by the Clinical and Laboratory Standards Institute (CLSI). The results showed that 19 fungi (8 genus) and 88 E. coli as well as 47 Salmonella sp. were obtained. The drug-resistant frequency of E. coil against all the tested antibiotics was 0%~35.55%. Of all the E. coli isolates, 35.22%, 12.50% and 12.50% were resistant to TET, AML and S3, respectively. Additionally, a total of 17 resistance profiles were observed, among which TET and AMP-TET were the dominant. In addition, 42.55%, 40.43% and 38.30% of Salmonella isolates were resistant to TET, AML and S3, respectively. Similarly, there were also 17 resistance profiles observed, and AML and AML-TET were the top two. Morphology and ITS sequencing showed that 19 species of fungi belonged to 8 different genus were isolated. The identification of the bacteria and fungi and the antimicrobial susceptibility testing could figure out the relationship between microorganism in intestinal tract and individuals of giant pandas. Moreover, this could facilitate the further study of the nutrition and digestion and providing basis for the enteric disease prevention, treatment and improvement of the livability of giant panda.