中华沙鳅(♀)与宽体沙鳅(♂)杂交种的胚胎发育
Embryonic Development of Hybrid of Sinibotia superciliaris (♀) and Botia reevesae (♂)
王永明1，谢碧文1*，岳兴建1，王芳1，齐泽民1，王淯1，吴英2
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DOI：
作者单位：(1.长江上游鱼类资源保护与利用四川省重点实验室，特色农业资源研究与利用四川省高校重点实验室，四川内江641112；2. 内江市第六中学，四川内江641000)
中文关键字：杂交种；胚胎发育；中华沙鳅；宽体沙鳅
英文关键字：hybrid; embryonic development; Sinibotia superciliaris; Botia reevesae
中文摘要：以中华沙鳅为母本，宽体沙鳅为父本，通过人工杂交获得受精卵，运用数码体视显微镜对杂交种的胚胎发育进行观察拍照，并对杂交种胚胎发育各时期特征进行了详细描述。受精卵直径1.41 mm±0.16 mm，吸水膨胀后直径达1.91 mm±0.09 mm，半漂流性。25℃±0.5℃水温条件下，受精卵历时25 h 50 min孵出。胚胎发育过程分为7个阶段、27个发育期。初孵仔鱼全长4.71 mm±0.12 mm。经统计，杂交种受精率46.84%，孵化率72.84%，仔鱼畸形率8.62%，表明这两种沙鳅属间杂交表现出亲和性。
英文摘要：The hybrids of Sinibotia superciliaris (♀) and Botia reevesae (♂) were obtained by artificial crossing, and the embryonic development of hybrids was observed using a microscope with digital camera. The fertilized floating eggs had a diameter of 1.41 mm±0.16 mm and would expand to 1.91 mm±0.09 mmafter water swelling. The eggs would drift in flowing water. The larvae would be hatched after fertilization for 25 h 50 min at water temperature of25℃±0.5℃. The embryonic development could be divided into 7 main periods including 27 developing stages. The total body length of newly hatched larvae was4.71 mm±0.12 mm. After calculation, the fertilization rate and hatching rate of embryo was about 46.84% and 72.84%, respectively. Only 8.62% of newly hatched larvae were abnormal. The results showed that the gametes from S. superciliaris and B. reevesae are compatible.