一种鱼类DNA的非损伤检测方法
A Method of Non-invasive Detection for Genome DNA of Fish
宋君1,2，王东2，叶先林2，雷绍荣2，郭灵安2
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DOI：
作者单位：(1. 四川大学生命科学学院，四川省濒危野生动物保护生物学重点实验室，成都 610064; 2. 四川省农业科学院分析测试中心，成都 610066)
中文关键字：非损伤性取样；粘液DNA；检测
英文关键字：non-invasive sampling; mucus DNA; detection
中文摘要：为了减少和改变对濒危野生鱼类的大量伤害性取样，本文探索了从非损伤性取样的鱼类体表粘液样品中提取鱼类基因组DNA的方法，并用常用的两种分子标记(Cyt b和D-Loop)检测了分离到的粘液DNA。结果表明，从非损伤性取样的鱼类粘液样品中能够分离到高质量的鱼类基因组DNA，可用于后续的研究工作。鱼类体表粘液的非损伤性取样及其DNA提取，为濒危野生鱼类遗传学研究提供了一种新的非损伤性DNA检测技术。
英文摘要： In order to reduce the damage and optimize the sampling from endangered wild fish, we developed an non-invasive sampling method from the mucus of fish surface. The DNA extracted from mucus was verified by using two popular genetic markers, Cyt b and D-Loop. The results showed the segments of Cyt b and D-Loop were well amplified, and the DNA isolated from the mucus of fish surface can be used for the subsequent analysis. Therefore, sampling and DNA extraction from mucus provide a new non-invasive detection technology for genetic research on endangered wild fish.