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溶菌酶和天蚕素对中华鳖腐皮病预防效果的研究
Study on Preventive Effect on Lysozyme and Cecropin of Skin Fester Disease of Trionyx sinensis
陈春山 郭明磊 魏凯 马龙 耿琰
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作者单位:北京市水生野生动植物救护中心,北京,102100
中文关键字:溶菌酶、天蚕素、中华鳖腐皮病、预防
英文关键字:lysozyme; cecropin; skin fester disease of Trionyx sinensis; prevention
中文摘要:为探讨以生物肽和生物抗生素替代抗生素对中华鳖腐皮病的防治效果。本实验采用肌肉注射天蚕素(10mg/kg)、溶菌酶(10mg/kg)及天蚕素(6mg/kg)和溶菌酶(5mg/kg)二联法,观察对鳗气单胞菌[请给出拉丁名](Aeromonas encheleia)引起的中华鳖腐皮病的预防效果。以死亡率和保护率评价不同药物的保护效果;通过检测血清凝集效价、细胞吞噬功能和血清杀菌活力,比较不同药物对中华鳖免疫力和抗病能力的影响;比较庆大霉素和溶菌酶血药残留浓度,评估药物毒副作用。实验结果表明:天蚕素和溶菌酶二联法对中华鳖保护效果最好、免疫效果最佳,与其他组相比均有显著差异(p<0.01);血药残留分析表明,溶菌酶用药后第6天全部代谢完毕,庆大霉素到第12天仍有残留。结论:用天蚕素和溶菌酶二联法预防中华鳖腐皮病效果显著优于抗生素的效果,无药物残留和耐药副作用,并提高免疫力,尤其是避免了应用抗生素所带来的危害,益于推广应用。
英文摘要:In order to study the preventive effect on peptides and biological antibiotics of skin fester disease of Trionyx sinensis, three different groups of lysozyme (10mg/kg), cecropin (10mg/kg) and the mixed drugs (6mg/kg and 5mg/kg, respectively) have been used for intramuscular injection and observe the preventive effect of skin fester disease of Trionyx sinensis caused by Aeromonas encheleia. On this basis, through calculating the mortality rate and the protection rate to evaluate the protective effects of different drugs. Detecting agglutination titer of serum, phagocytosis and bactericidal activity of serum to compare the effects of different drugs on immunity and disease resistance of Trionyx sinensis. Comparing the residual concentration of gentamicin and lysozyme to evaluate drug toxic and side effects. The results indicated that the group of mixed drugs had a significant difference(P<0.01), which was the most effective for protection and immunity of Trionyx sinensis. Analysis of drug residue showed that lysozyme finished the whole metabolism 6 days later, in contrast, gentamicin still had residue until Day 12. Therefore, the preventive effect of mixed drugs is significantly better than antibiotics in skin fester disease of Trionyx sinensis. In addition, mixed drugs of lysozyme and cecropin are worthy of spreading to application in virtue of no drug residue and other side effects, especially avoiding the damage caused by abuse of antibiotics.
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国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
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